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Series GSE134358 Query DataSets for GSE134358
Status Public on Jan 11, 2021
Title Small non-coding RNA expression in whole blood leukocytes of healthy subjects, critically-ill patients with sepsis or non-infectious etiology and human endotoxemia
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Expression profiling by array
Summary Genome-wide gene expression profiling of whole blood leukocytes in critically ill patients with sepsis or non-infectious disease has been used extensively in search of diagnostic biomarkers, as well as prognostic signatures reflecting diseases severity and outcome. Through technological advances in genomics it has become clear that transcription is not limited to protein-coding regions of the genome. Here, we describe a comprehensive analysis of small non-coding RNA expression in blood leukocytes of critically ill patients with sepsis, a non-infectious condition, healthy subjects and experimental human endotoxemia
 
Overall design Whole-blood transcriptional patterns of small non-coding RNA molecules in healthy subjects, critically-ill patients with sepsis or non-infectious conditions and experimental human endotoxemia (2ng/kg Escherichia coli lipopolysaccharide (LPS)) were generated by means of the Affymetrix Multispecies miRNA-4_1 Array. Whole-blood from patients was collected within 24 hours of intensive care unit (ICU) admission. Whole blood of healthy male volunteers who participated in a human endotoxemia study was collected before and 2, 4, 6 and 24 hours after intravenous LPS administration. The Dutch Central Committee on Research Involving Human Subjects (CCMO) and the Medical Ethics Committee of the Academic Medical Center (AMC), Amsterdam, the Netherlands, approved the study. Title legend: healthy, healthy subjects; sepsis, ICU patients with sepsis; noninfectious, ICU patients with non-infectious condition; endo_0h, healthy subjects before LPS administration; endo_2h, endo_4h, endo_6h, endo_24h, healthy subjects after 2, 4, 6 and 24 hours LPS administration, respectively.
 
Contributor(s) Scicluna BP, van der Poll T
Citation(s) 33305733
Submission date Jul 16, 2019
Last update date Jan 11, 2021
Contact name Brendon Scicluna
E-mail(s) brendon.scicluna@um.edu.mt
Organization name University of Malta
Street address Msida campus
City Msida
ZIP/Postal code MSD2020
Country Malta
 
Platforms (1)
GPL21572 [miRNA-4] Affymetrix Multispecies miRNA-4 Array [ProbeSet ID version]
Samples (567)
GSM3943133 hv190
GSM3943134 hv186
GSM3943135 hv198
This SubSeries is part of SuperSeries:
GSE134364 Protein-coding and non-coding RNA landscape in critically ill patients with sepsis
Relations
BioProject PRJNA554860

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE134358_RAW.tar 418.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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