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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 01, 2009 |
| Title |
Adipose tissue exposed to cold |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Cold triggers VEGF dependent but hypoxia independent angiogenesis in adipose tissues and anti-VEGF agents modulate adipose metabolism
The molecular mechanisms of angiogenesis in relation to adipose tissue metabolism remain poorly understood. Here we show that exposure of mice to cold led to conversion of white adipose tissue (WAT) to brown-like adipose tissue, accompanying the switch of an active angiogenic phenotype. Gene expression profile analysis showed VEGF was upregulated via most likely hypoxia-independent PGC-1 transcriptional activation. Intriguingly, VEGFR2 blockage abolished the cold-induced angiogenesis, significantly impaired nonshivering thermogenesis capacity, and markedly reduced adipose metabolism. Unexpectedly, VEGFR1 blockage resulted in opposite effects by increasing adipose vascularity and metabolism. These findings demonstrate that VEGFR2 and VEGFR1 mediate polarized activities in modulating adipose angiogenesis and metabolism. Taken together, our findings have conceptual implications in applying angiogenesis modulators for the treatment of obesity and metabolic disorders.
Keywords: Time course
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| Overall design |
Mice were exposed to cold and white addipose tissue was collected at different time points
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| Contributor(s) |
Larsson O, Cao Y |
| Citation(s) |
19117550, 23823482 |
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| Submission date |
Nov 01, 2008 |
| Last update date |
Aug 23, 2019 |
| Contact name |
Ola Larsson |
| E-mail(s) |
ola.larsson@ki.se
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| Phone |
+46 (0)8 517 73280
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| Organization name |
Karolinska Institutet
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| Department |
Department of oncology-pathology
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| Street address |
CCK, R8:01
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| City |
Stockholm |
| ZIP/Postal code |
171 76 |
| Country |
Sweden |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA110017 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE13432_RAW.tar |
48.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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