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Status |
Public on Dec 05, 2019 |
Title |
Single-cell RNA-sequencing of spleen memory CD4+ T cells |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
We performed a single-cell transcriptome analysis of 3 biological independent splenic samples from pooled mice sorted for LCMV-specific memory CD4 T cells.
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Overall design |
Cells negative for dump and positive for CD4, CD44 and gp66 were sorted for each replicate, and used for single cell RNA Seq (10x genomics platform)
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Contributor(s) |
Kuenzli M, Schreiner D, Roux J, King C |
Citation(s) |
32144185, 35829695 |
Submission date |
Jul 11, 2019 |
Last update date |
Aug 02, 2022 |
Contact name |
DBM Bioinformatics Core Facility |
Phone |
+41612073541
|
Organization name |
University of Basel
|
Department |
Departement of Biomedicine
|
Street address |
Hebelstrasse 20
|
City |
Basel |
State/province |
BS |
ZIP/Postal code |
4053 |
Country |
Switzerland |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (3) |
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This SubSeries is part of SuperSeries: |
GSE139198 |
Long-lived T follicular helper cells retain plasticity and help sustain humoral immunity |
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Relations |
BioProject |
PRJNA554108 |
SRA |
SRP214289 |