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Series GSE134136 Query DataSets for GSE134136
Status Public on Aug 09, 2019
Title EZH2 inhibition results in genome-wide PRC2 redistribution in cancer cell models
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Upon EZH2 inhibitor treatment, PRC2 components EZH2 and SUZ12 are redistributed across the genome towards a small number of genomic loci that are refractory to H3K27me3 loss. Focal PRC2 accumulation and H3K27me3 retention occur primarily at CpG islands and transcription start sites of genes, with enrichment at canonical PRC2 nucleation sites. EZH2 and SUZ12 are present at low levels at actively transcribed TSSs, and this signal is lost with EZH2 inhibition.
 
Overall design ChIP-seq of PRC2 components, H3K27me3, and H3K4me3 upon treatment with EZH2 inhibitor or a control, in DLBCL and multiple myeloma cell lines.
 
Contributor(s) Jeon A, Bryant B, Trojer P, Tucker-Kellogg G, Yuan C
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jul 11, 2019
Last update date Aug 11, 2019
Contact name Barbara M Bryant
E-mail(s) barbara.bryant@constellationpharma.com
Organization name Constellation Pharmaceuticals
Street address 215 First Street
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (44)
GSM3937774 KARPAS-422 DMSO 8 days Input ChIP-seq
GSM3937775 KARPAS-422 DMSO 8 days H3K27ac ChIP-seq
GSM3937776 KARPAS-422 DMSO 8 days EZH2 ChIP-seq
Relations
BioProject PRJNA554018
SRA SRP214208

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE134136_RAW.tar 16.2 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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