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Series GSE13411 Query DataSets for GSE13411
Status Public on Dec 31, 2008
Title Gene expression by human splenic B-cell subsets
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Enhanced secondary Ab responses are a vital component of adaptive immunity, yet little is understood about the intrinsic and extrinsic regulators of naïve and memory B cells that results in differences in their responses to Ag. Microarray analysis, together with surface and intracellular phenotyping, revealed that memory B cells have increased expression of members of the TNF receptor, SLAM, B7 and Bcl2 families, as well as the TLR-related molecule CD180 (RP105). Accordingly, memory B cells exhibited enhanced survival, proliferation and Ig secretion, as well as entered division more rapidly than naïve B cells in response to both T-dependent and T-independent stimuli. Furthermore, both IgM and isotype switched memory B cells, but not naïve B cells, co-stimulated CD4+ T cells in vitro through a mechanism dependent on their constitutive expression of CD80 and CD86. This study demonstrates that upregulation of genes involved in activation, co-stimulation and survival provides memory B cells with a unique ability to produce enhanced immune responses and contributes to the maintenance of the memory B cell pool.

Keywords: cell type comparison
 
Overall design Four subsets of human splenic B cells (naïve, IgM-memory, Ig isotype switched memory and plasma cells); sort-purified and analysed immediately ex vivo; performed in duplicate.
 
Contributor(s) Good KL, Tangye SG
Citation(s) 19124732
Submission date Oct 30, 2008
Last update date Aug 10, 2018
Contact name Stuart Tangye
E-mail s.tangye@garvan.org.au
Phone +61 2 9295 8455
Organization name Garvan Institute of Medical Research
Department Immunology
Street address 384 Victoria St
City Darlinghurst
State/province NSW
ZIP/Postal code 2010
Country Australia
 
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (8)
GSM338401 naïve B cells, rep 1
GSM338402 naïve B cells, rep 2
GSM338403 IgM memory B cells, rep 1
Relations
BioProject PRJNA109773

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Supplementary file Size Download File type/resource
GSE13411_RAW.tar 24.4 Mb (http)(custom) TAR (of CEL)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table

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