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| Status |
Public on Jul 11, 2019 |
| Title |
MicroRNA profiling during neural differentiation of induced pluripotent stem cells |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
MicroRNAs (miRNA) play an essential role in the regulation of gene expression, influence signaling networks responsible for several cellular processes like differentiation of pluripotent stem cells. Despite several studies on the neurogenesis process, no global analysis of microRNA expression during differentiation of induced pluripotent stem cells (iPSC) to neuronal stem cells (NSC) has been done. Therefore we compared the profile of microRNA expression in iPSC lines and in NSC lines derived from them, using microarray-based analysis. Two different protocols for NSC formation were used: direct and two-step via neural rosette formation. We confirmed the new associations of previously described miRNAs in regulation of NSC differentiation from iPSC. We discovered upregulation of miR-10 family, miR-30 family and miR-9 family and downregulation of miR-302 and miR-515 family. Moreover we showed that miR-10 family play a crucial role in the negative regulation of genes expression belonging to signaling pathways involved in neural differentiation: WNT signaling pathway, focal adhesion, signaling pathways regulating pluripotency of stem cells.
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| Overall design |
We conducted differentiation of human induced pluripotent stem cells into neural stem cells. Then we carried out global miRNA expression analysis using the Affymetrix platform. We profiled the miRNAs which are crucial regulators of neural differentiation. We not only confirmed the involvement of previously described miRNAs characteristic for transition from ES to NSC: mir-302 family, hsa-miR-125b, hsa-miR-9, let-7, but we also pointed out others, which may be characteristic for iPSC to NSC transition.
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| Contributor(s) |
Kulcenty K, Wroblewska JP, Rucinski M, Kozlowska E, Jopek K, Suchorska WM |
| Citation(s) |
31357387 |
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| Submission date |
Jul 10, 2019 |
| Last update date |
Sep 11, 2019 |
| Contact name |
Marcin Rucinski |
| E-mail(s) |
marcinruc@ump.edu.pl
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| Organization name |
Poznan University of Medical Science
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| Department |
Histology and Embriology
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| Street address |
Swiecicki 6
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| City |
Poznan |
| ZIP/Postal code |
60-781 |
| Country |
Poland |
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| Platforms (1) |
| GPL19117 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array |
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| Samples (8)
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| GSM3935825 |
undifferentiated GPCCi001-A cell line, biol. rep 1 |
| GSM3935826 |
undifferentiated ND41658*H cell line, biol. rep 1 |
| GSM3935827 |
undifferentiated GPCCi001-A cell line, biol. rep 2 |
| GSM3935828 |
undifferentiated ND41658*H cell line, biol. rep 2 |
| GSM3935829 |
neuronal stem cells differentiated from GPCCi001-A cell line,biol. rep 1 |
| GSM3935830 |
neuronal stem cells differentiated from GPCCi001-A cell line,biol. rep 2 |
| GSM3935831 |
neuronal stem cells differentiated from ND41658*H cell line,biol. rep 1 |
| GSM3935832 |
neuronal stem cells differentiated from ND41658*H cell line,biol. rep 2 |
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| Relations |
| BioProject |
PRJNA553662 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE134061_RAW.tar |
6.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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