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Series GSE13394 Query DataSets for GSE13394
Status Public on Nov 14, 2008
Title A translational profiling approach for the molecular characterization of CNS cell types
Organism Mus musculus
Experiment type Expression profiling by array
Summary The cellular heterogeneity of the brain confounds efforts to elucidate the biological properties of distinct neuronal populations.
Using Bacterial Artificial Chromosome (BAC) transgenic mice which express EGFP-tagged ribosomal protein L10a in defined cell populations, we have developed a methodology to affinity purify polysomal mRNAs from genetically defined cell populations in the brain.
The utility of this approach is illustrated by the comparative analysis of four types of neurons, revealing hundreds of genes that distinguish these four cell populations.
We find that even two morphologically indistinguishable, intermixed subclasses of medium spiny neuron display vastly different translational profiles and present examples of the physiological significance of such differences.
This genetically targeted Translating Ribosome Affinity Purification (TRAP) methodology is a generalizable method useful for the identification of molecular changes in any genetically defined cell type in response to genetic alterations, disease, or pharmacological perturbations.

This SuperSeries is composed of the SubSeries listed below.
 
Overall design Refer to individual Series
 
Contributor(s) Heiman M, Schaefer A, Gong S, Peterson J, Day M, Ramsey KE, Suárez-Fariñas M, Schwarz C, Stephan DA, Surmeier DJ, Greengard P, Heintz N
Citation(s) 19013281
Submission date Oct 29, 2008
Last update date Feb 11, 2019
Contact name Nathaniel Heintz
E-mail heintz@rockefeller.edu
Organization name HHMI/Rockefeller University
Street address 1230 York Ave, Box 260
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (63)
GSM338145 D1_IP1: Drd1a Medium spiny neurons, dissected mouse striatum
GSM338146 D1_IP2: Drd1a Medium spiny neurons, dissected mouse striatum
GSM338147 D1_IP3: Drd1a Medium spiny neurons, dissected mouse striatum
This SuperSeries is composed of the following SubSeries:
GSE13384 Comparative analysis of Drd1+ Medium Spiny Neurons and Drd2+ Medium Spiny Neurons
GSE13385 Comparative analysis of Drd1+ Medium Spiny Neurons, Drd2+ Medium Spiny Neurons and whole brain
GSE13386 Comparative analysis of Drd1+ Medium Spiny Neurons, Drd2+ Medium Spiny Neurons, cocaine treatment
Relations
BioProject PRJNA109821

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13394_RAW.tar 270.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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