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| Status |
Public on Aug 21, 2019 |
| Title |
Single-cell atlas of thalamic projection systems |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We collected single-cell RNAseq data from neurons of five thalamocortical projection systems (motor, somatosensory, visual, auditory, and prefrontal). Cells of each projection system were retrogradely labeled from their cortical target area, microdissected, and collected individually. By combining single-cell transcriptomics with in situ RNA hybridization, we found heterogeneity not only between thalamic nuclei but also within nuclei, with graded transitions between cell identities.
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| Overall design |
Single-cell transcriptome profiling of 5 thalamocortical projection systems, defined via retrograde labeling
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| Contributor(s) |
Schulmann A, Phillips JW, Hara E, Wang L, Lemire AL, Nelson SB, Hantman AW |
| Citation(s) |
31527803 |
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| Submission date |
Jul 06, 2019 |
| Last update date |
Nov 26, 2019 |
| Contact name |
Anton Schulmann |
| E-mail(s) |
anton.schulmann@nih.gov
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| Organization name |
NIH/NIMH
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| Lab |
McMahon
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| Street address |
35 Convent Dr
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| City |
Bethesda |
| State/province |
Maryland (MD) |
| ZIP/Postal code |
20814 |
| Country |
USA |
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| Platforms (1) |
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| Samples (26)
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| Relations |
| BioProject |
PRJNA552997 |
| SRA |
SRP213360 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE133912_thal_singlecell_counts.csv.gz |
16.5 Mb |
(ftp)(http) |
CSV |
| GSE133912_thal_singlecell_metadata.csv.gz |
42.9 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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