|
| Status |
Public on Nov 21, 2019 |
| Title |
Transcriptome analyses of human pancreatic islets and pseudoislets |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have observed an improvement of glucose-stimulated insulin secretion upon the formation of pseudoislets. Transcriptome analyses of islets and pseudoislets from the same human donor were performed to determine the functional improvement. We identified 38844 transcripts and revealed that unlike islets, pseudoislets were deprived of exocrine and endothelial cells. In addition, the mRNA levels of proteins related to apoptosis and inflammation as well as the components of extracellular matrix were less abundant in pseudoislets.
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| |
|
| Overall design |
human islets preparations and pseudoislets from 4 different human donors
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| |
|
| Contributor(s) |
Ullrich S, Lorza-Gil E, Dahl A, Czemmel S, Gauder M |
| Citation(s) |
31311971 |
| |
| Submission date |
Jul 06, 2019 |
| Last update date |
May 11, 2021 |
| Contact name |
Stefan Czemmel |
| Organization name |
Quantitative Biology Center (QBiC)
|
| Department |
University of Tuebingen
|
| Street address |
Auf der Morgenstelle 10
|
| City |
Tuebingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (9)
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| Relations |
| BioProject |
PRJNA552979 |
| SRA |
SRP213348 |
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