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Series GSE13379 Query DataSets for GSE13379
Status Public on Nov 14, 2008
Title Application of a translational profiling approach for the comparative analysis of CNS cell types.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Comparative analysis can provide important insights into complex biological systems. As demonstrated in the accompanying paper, Translating Ribosome Affinity Purification (TRAP), permits comprehensive studies of translated mRNAs in genetically defined cell populations following physiological perturbations.
To establish the generality of this approach, we present translational profiles for twenty four CNS cell populations, and identify known cell-specific and enriched transcripts for each population.
We report thousands of cell-specific mRNAs that were not detected in whole tissue microarray studies, and provide examples that demonstrate the benefits deriving from comparative analysis.
To provide a foundation for further biological and in silico studies, we provide a resource of sixteen transgenic mouse lines, their corresponding anatomic characterization, and translational profiles for cell types from a variety of CNS structures.
This resource will enable a wide spectrum of molecular and mechanistic studies of both well known and previously uncharacterized neural cell populations.

Keywords: Cell Type Comparison
 
Overall design For each cell population, three independent TRAP replicates were collected, and total RNA from both the immunoprecipitate and unbound fractions were seperately amplified and hybridized.
For each tissue, several representative unbound fractions are provided to serve as controls. Biological replicates are GCRMA normalized within groups.
Following averaging of replicates, we recommend further global normalization between groups, using affymetrix biotinylated controls, to correct for any broad biases in scanning and hybridization.
Finally for many analyses, we also recommend filtering to remove those probesets with low IP/UB fold change values from each cell type.
Researchers can contact us for spreadsheets where these additional steps have been completed.
 
Contributor(s) Doyle JP, Dougherty JD, Heiman M, Schmidt EF, Stevens TR, Ma G, Bupp S, Shrestha P, Shah RD, Doughty ML, Gong S, Greengard P, Heintz N
Citation(s) 19013282, 20308160
Submission date Oct 28, 2008
Last update date Feb 11, 2019
Contact name Nathaniel Heintz
E-mail heintz@rockefeller.edu
Organization name HHMI/Rockefeller University
Street address 1230 York Ave, Box 260
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (107)
GSM337760 Astrocytes, Cerebellum, TRAP RNA 1
GSM337761 Astrocytes, Cerebellum, TRAP RNA 2
GSM337762 Astrocytes, Cerebellum, TRAP RNA 3
Relations
BioProject PRJNA109803

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13379_RAW.tar 640.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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