 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 26, 2019 |
| Title |
Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Sertoli cells are essential nurse cells in the testis that regulate the process of spermatogenesis and establish the immune-privileged environment of the blood-testis-barrier (BTB). The induction of human Sertoli cells from fibroblasts could provide cellular sources for fertility and transplantation treatments. Here, we report the in vitro reprogramming of human fibroblasts to Sertoli cells and characterize these human induced Sertoli-like cells (hiSCs). Initially, five transcriptional factors (NR5A1, GATA4, WT1, SOX9 and DMRT1) and a gene reporter carrying the AMH promoter were utilized to obtain the hiSCs. We further reduce the number of reprogramming factors to two, i.e., NR5A1 and GATA4, and show that these hiSCs have transcriptome profiles that are similar to those of primary human Sertoli cells. Consistent with the known cellular properties of Sertoli cells, hiSCs attract endothelial cells and exhibit high number of lipid droplets in the cytoplasm. More importantly, hiSCs can sustain the viability of spermatogonia cells harvested from mouse seminiferous tubules. In addition, hiSCs suppress the production of IL-2 and proliferation of human T lymphocytes. When hiSCs were cotransplanted with human embryonic kidney cells, these xenotransplanted human cells survived longer in mice with normal immune systems. hiSCs also allow us to determine a gene associated with Sertoli-only syndrome (SCO), CX43, is indeed important in regulating the maturation of Sertoli cells.
|
| |
|
| Overall design |
Examination of whole transcriptome in 7 cell types.
|
| |
|
| Contributor(s) |
Liang J, Wang N, He J, Du J, Kee K |
| Citation(s) |
31710289 |
| |
| Submission date |
Jul 03, 2019 |
| Last update date |
Nov 26, 2019 |
| Contact name |
Jianlin Liang |
| E-mail(s) |
jianlin.liang@foxmail.com
|
| Phone |
86-13020007968
|
| Organization name |
Tsinghua University
|
| Department |
Medical School
|
| Lab |
Stem cell and reproduction
|
| Street address |
Tsinghua park
|
| City |
Beijing |
| ZIP/Postal code |
100086 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (14)
|
|
| Relations |
| BioProject |
PRJNA552468 |
| SRA |
SRP212868 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE133757_RAW.tar |
1.6 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...