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Series GSE133756 Query DataSets for GSE133756
Status Public on Jul 04, 2019
Title Targeted RNA-sequencing Facilitates Quantitative Analysis of RB and E2F3 in mouse Sertoli cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary A Sertoli cell specific knockout of retinoblastoma protein (SC-RbKO) results in a complete disruption of spermatogenesis in mice. In this project the SC-RbKO mice were cross-bred with E2F3 floxed mice to see if a double knockout would rescue the phenotype associated with RB loss.
 
Overall design Gene expression analysis by Targeted RNA-sequencing between SC-E2F3KO and Wild type at 2 and 5 months old testes and among SC-Rb-/-E2F3+/+, SC-Rb-/-E2F3+/-, SC-Rb-/-E2F3-/- and Wild type at 2,5 months old testes were performed.
 
Contributor(s) Rotgers E
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jul 03, 2019
Last update date Jul 07, 2019
Contact name Emmi Rotgers
E-mail(s) esrotg@utu.fi
Organization name University of Turku
Department Institute of Biomedicine
Lab Jorma Toopari┬┤s lab
Street address Kiinamyllynkatu 10
City Turku
ZIP/Postal code 20520
Country Finland
 
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (44)
GSM3926664 Testis, b.24.8 6.
GSM3926665 Testis, b. 6.10 3.
GSM3926666 Testis, b. 6.10. 2
Relations
BioProject PRJNA552469
SRA SRP212867

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE133756_2.5_months_SC_RB_E2F3_NormalizedHitsPerSample_M1.tsv.gz 20.0 Kb (ftp)(http) TSV
GSE133756_2.5_months_SC_RB_E2F3_TargetHitsPerSample_M1.json.gz 6.8 Kb (ftp)(http) JSON
GSE133756_2_months_SC_E2F3_NormalizedHitsPerSample_M1.tsv.gz 9.5 Kb (ftp)(http) TSV
GSE133756_2_months_SC_E2F3_TargetHitsPerSample_M1.json.gz 4.1 Kb (ftp)(http) JSON
GSE133756_5_months_SC_E2F3_NormalizedHitsPerSample_M1.tsv.gz 9.6 Kb (ftp)(http) TSV
GSE133756_5_months_SC_E2F3_TargetHitsPerSample_M1.json.gz 4.1 Kb (ftp)(http) JSON
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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