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Series GSE133624 Query DataSets for GSE133624
Status Public on Jul 02, 2019
Title Transcriptomics analysis of gene expression in human urothelial carcinoma of the bladder (UCB) and adjacent normal tissues.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary 36 cases of UCB and 29 adjacent normal bladder tissues (22 pairs) were derived from patients diagnosed with UCB and received radial cystectomy at Sun Yat-sen University Cancer Center (SYSUCC, Guangzhou, China). Total RNA was isolated using TRIzol® Reagent (Ambion). The RNA concentration and quality were determined with NanoDrop, Qubit and agarose gel analysis. The Dynabeads® mRNA Purification Kit (Ambion) was used to enrich the mRNAs, which were used as templates for RNA-Seq library construction. RNA-Seq libraries was directly constructed by using KAPA mRNA Stranded mRNA-Seq Kit (KAPA) according to the manufacturer’s instructions. The libraries were sequenced on the Illumina HiSeq X-Ten platform at Novogene (Tianjin, CA) with paired-end 150 bp read length. The different genes expression between the UCB and adjacent normal tissues were detected. Several genes related to cancer pathways were dysregulated.
Overall design Examination of gene expressive change in human urothelial carcinoma of the bladder (UCB)
Contributor(s) Chen X, Li A, Sun B, Xie D, Yang Y
Citation(s) 31358969
Submission date Jul 01, 2019
Last update date Apr 10, 2020
Contact name Bao-Fa Sun
Organization name Beijing Institute of Genomics (BIG) of Chinese Academy of Sciences (CAS)
Street address Da-Tun Road
City Beijing
ZIP/Postal code 100101
Country China
Platforms (1)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (65)
GSM3913398 N0156-T
GSM3913399 N0453-T
GSM3913400 N0466-T
This SubSeries is part of SuperSeries:
GSE133671 m5C Promotes Pathogenesis of the Bladder Cancer Through Stabilizing mRNAs
BioProject PRJNA552055
SRA SRP212702

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Supplementary file Size Download File type/resource
GSE133624_reads-count-all-sample.txt.gz 2.9 Mb (ftp)(http) TXT
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