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| Status |
Public on Jun 22, 2020 |
| Title |
RNA seq to compare gene expression profiles between Npm1 WT and Npm1 knockdown cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Mouse KrasG12D/P53-/-(KP) lung tumor cells were transfected with pLKO.1-Tet on-shNpm1-3(N3), pLKO.1-Tet on-shNpm1-4(N4) or pLKO.1-Tet on-shNpm1-5 (N5) and then selected by using 2ug/ml puromycin, to establish the 3 stable cell lines. These 3 stable cell lines (N3, N4 or N5) were treated with 100ng/ml Doxycline and then the total RNA of the treated and untreated cells were harvested for RNA seq.
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| |
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| Overall design |
Examination of differential gene expression profils in KP-shNpm1-3 (N3), KP-shNpm1-4 (N4) and KP-shNpm1-5 (N5) cells with or without Doxycycline treatment
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| |
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| Contributor(s) |
Li F, Khodadadi-Jamayran A, Wong K |
| Citation(s) |
32646968 |
| |
| Submission date |
Jun 30, 2019 |
| Last update date |
Sep 21, 2020 |
| Contact name |
Alireza Khodadadi-Jamayran |
| Organization name |
New York University, NYU Langone Medical Center
|
| Department |
Division of Advanced Research Technologies (DART)
|
| Lab |
Applied Bioinformatics Laboratories (ABL)
|
| Street address |
550 1st Ave, MSB 304
|
| City |
New York City |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA551883 |
| SRA |
SRP212657 |
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