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| Status |
Public on Jul 02, 2020 |
| Title |
Matched primary ovarian cancer, omental metastases, and non-omental metastases from 10 patients with high-grade serous ovarian carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Matched high-grade serous ovarian carcinoma samples collected from the ovary (ov), omental metastasis (om-met), and non-omental intraperitoneal metastasis (met) from 10 patients at the time of primary debulking surgery were analyzed for RNA expression by RNA sequencing.
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| Overall design |
FFPE blocks of matched high-grade serous ovarian carcinoma samples collected from the ovary (ov), omental metastasis (om-met), and non-omental intraperitoneal metastasis (met) from 10 patients were sectioned onto uncharged glass slides. One 4 µm H&E-stained section was used by a pathologist to circle the tumor areas and delineate them from the adjacent normal tissue. Depending on the tumor size, 1 to 3 unstained 10 µm sections were macrodissected (removal of non-tumor areas based on the H&E template) with a clean razor blade. Total RNA was isolated using the miRNeasy FFPE kit. RNA sequencing was done by MedGenome.
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| Contributor(s) |
Hu Y, Taylor-Harding B, Orsulic S |
| Citation(s) |
32766252 |
| |
| Submission date |
Jun 25, 2019 |
| Last update date |
Oct 01, 2020 |
| Contact name |
Ye Hu |
| E-mail(s) |
huye327@126.com
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| Phone |
3236129425
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| Organization name |
Cedars Sinai Medical Center
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| Street address |
8635 W. 3rd Street, Suite 290W
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| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90048 |
| Country |
USA |
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| Platforms (1) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA551007 |
| SRA |
SRP212075 |
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