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| Status |
Public on Aug 26, 2020 |
| Title |
Cryptococcus neoformans reactivation in different media and temperatures |
| Organism |
Cryptococcus neoformans H99 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We wanted to find out the transcriptomic response of growth-arrested Cryptococcus yeast cells when initially placed in environments in which they could reactivate or "wake up", focusing on the difference between a rich microbial media (YPD) and a common cell culture media, RPMI + 10% heat-inactivated fetal calf serum.
Please contact Edward.Wallace@ed.ac.uk if you use this data and ask for citation information; we are currently (August 2020) working on a publication.
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| Overall design |
36-sample RNA-seq timecourse of reactivation of growth-arrested Cryptococcus neoformans H99 in two media at two temperatures. Two biological samples of cells were pre-grown for 5 days in 10mL YPD. A sample of this was taken as the "0 minute" control timepoint. We then inoculated to OD 0.1 in 4 different cultures of 100mL pre-warmed media: (YPD, RPMI+) x (25C, 37C), and grew for 10, 30, 60, or 120 min. We also obtained a timepoint in more standard growth conditions of YPD, 30C, 180mins.
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| Web link |
https://ewallace.github.io/
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| |
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| Contributor(s) |
Wallace E, Ballou E |
| Citation(s) |
37398450, 40464551 |
| |
| Submission date |
Jun 20, 2019 |
| Last update date |
Jul 03, 2025 |
| Contact name |
Edward William Joseph Wallace |
| E-mail(s) |
edward.wallace@ed.ac.uk
|
| Organization name |
University of Edinburgh
|
| Department |
Institute for Cell Biology, School of Biological Sciences
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| Street address |
Waddington Building, Max Born Crescent
|
| City |
Edinburgh |
| ZIP/Postal code |
EH9 3BF |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL24689 |
Illumina NextSeq 500 (Cryptococcus neoformans var. grubii H99) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA549871 |
| SRA |
SRP201981 |
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