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Status |
Public on Jun 18, 2019 |
Title |
Transcriptome profiling of abiotic responses to heat, cold, salt, and osmotic stress of Capsicum annuum L. |
Organism |
Capsicum annuum |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We sought to investigate the scope of transcriptome analyses of peppers subjected to four major environment stresses (heat, cold, drought, and salinity). For this, at the six-true-leaf stage, plants were subjected to a temperature of 10°C or 40°C to mimic cold or heat stress, respectively. For salinity stress, plants were treated with 50 mL of a 400 mM NaCl solution; for osmotic stress, the peppers were treated with 50 mL of 400 mM mannitol. For RNA-seq library construction, the third or fourth leaves from four plants were harvested per replicate at 0, 3, 6, 12, 24, and 72 h after treatment. A total of 204.68 Gb of transcriptome data were generated using transcriptome analysis pipelines consisting of quality control, quantification, and differential gene expression analyses. A hierarchical clustering of gene expression data were used to infer the quality of the RNA-seq data and the characteristics of samples in each treatment. The extensive transcriptome data obtained will provide valuable information for future studies of crops exposed to abiotic stresses.
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Overall design |
RNA-Seq profiling of abiotic responses to heat, cold, salt, and osmotic stress in Capsicum annuum L. leaves
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Contributor(s) |
Kang W, Sim Y, Koo N, Nam J, Lee J, Kim N, Kim Y, Yeom S |
Citation(s) |
31932587 |
BioProject |
PRJNA525913 |
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Submission date |
Jun 17, 2019 |
Last update date |
Jan 27, 2020 |
Contact name |
Seon-In Yeom |
E-mail(s) |
sunin78@gnu.ac.kr
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Organization name |
Gyeongsang National University
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Street address |
501, Jinjou-daero
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City |
Jinju |
ZIP/Postal code |
52828 |
Country |
South Korea |
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Platforms (1) |
GPL26789 |
Illumina HiSeq 2500 (Capsicum annuum) |
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Samples (78)
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GSM3893038 |
Leaves, Abio-mock-3h-1 |
GSM3893039 |
Leaves, Abio-mock-3h-2 |
GSM3893040 |
Leaves, Abio-mock-3h-3 |
GSM3893041 |
Leaves, Abio-mock-6h-1 |
GSM3893042 |
Leaves, Abio-mock-6h-2 |
GSM3893043 |
Leaves, Abio-mock-6h-3 |
GSM3893044 |
Leaves, Abio-mock-12h-1 |
GSM3893045 |
Leaves, Abio-mock-12h-2 |
GSM3893046 |
Leaves, Abio-mock-12h-3 |
GSM3893047 |
Leaves, Abio-mock-24h-1 |
GSM3893048 |
Leaves, Abio-mock-24h-2 |
GSM3893049 |
Leaves, Abio-mock-24h-3 |
GSM3893050 |
Leaves, Abio-mock-72h-1 |
GSM3893051 |
Leaves, Abio-mock-72h-2 |
GSM3893052 |
Leaves, Abio-mock-72h-3 |
GSM3893053 |
Leaves, Cold-3h-1 |
GSM3893054 |
Leaves, Cold-3h-2 |
GSM3893055 |
Leaves, Cold-3h-3 |
GSM3893056 |
Leaves, Cold-6h-1 |
GSM3893057 |
Leaves, Cold-6h-2 |
GSM3893058 |
Leaves, Cold-6h-3 |
GSM3893059 |
Leaves, Cold-12h-1 |
GSM3893060 |
Leaves, Cold-12h-2 |
GSM3893061 |
Leaves, Cold-12h-3 |
GSM3893062 |
Leaves, Cold-24h-1 |
GSM3893063 |
Leaves, Cold-24h-2 |
GSM3893064 |
Leaves, Cold-24h-3 |
GSM3893065 |
Leaves, Cold-72h-1 |
GSM3893066 |
Leaves, Cold-72h-2 |
GSM3893067 |
Leaves, Cold-72h-3 |
GSM3893068 |
Leaves, Heat-3h-1 |
GSM3893069 |
Leaves, Heat-3h-2 |
GSM3893070 |
Leaves, Heat-3h-3 |
GSM3893071 |
Leaves, Heat-6h-1 |
GSM3893072 |
Leaves, Heat-6h-2 |
GSM3893073 |
Leaves, Heat-6h-3 |
GSM3893074 |
Leaves, Heat-12h-1 |
GSM3893075 |
Leaves, Heat-12h-2 |
GSM3893076 |
Leaves, Heat-12h-3 |
GSM3893077 |
Leaves, Heat-24h-1 |
GSM3893078 |
Leaves, Heat-24h-2 |
GSM3893079 |
Leaves, Heat-24h-3 |
GSM3893080 |
Leaves, Heat-72h-1 |
GSM3893081 |
Leaves, Heat-72h-2 |
GSM3893082 |
Leaves, Heat-72h-3 |
GSM3893083 |
Leaves, NaCl-3h-1 |
GSM3893084 |
Leaves, NaCl-3h-2 |
GSM3893085 |
Leaves, NaCl-3h-3 |
GSM3893086 |
Leaves, NaCl-6h-1 |
GSM3893087 |
Leaves, NaCl-6h-2 |
GSM3893088 |
Leaves, NaCl-6h-3 |
GSM3893089 |
Leaves, NaCl-12h-1 |
GSM3893090 |
Leaves, NaCl-12h-2 |
GSM3893091 |
Leaves, NaCl-12h-3 |
GSM3893092 |
Leaves, NaCl-24h-1 |
GSM3893093 |
Leaves, NaCl-24h-2 |
GSM3893094 |
Leaves, NaCl-24h-3 |
GSM3893095 |
Leaves, NaCl-72h-1 |
GSM3893096 |
Leaves, NaCl-72h-2 |
GSM3893097 |
Leaves, NaCl-72h-3 |
GSM3893098 |
Leaves, Man-3h-1 |
GSM3893099 |
Leaves, Man-3h-2 |
GSM3893100 |
Leaves, Man-3h-3 |
GSM3893101 |
Leaves, Man-6h-1 |
GSM3893102 |
Leaves, Man-6h-2 |
GSM3893103 |
Leaves, Man-6h-3 |
GSM3893104 |
Leaves, Man-12h-1 |
GSM3893105 |
Leaves, Man-12h-2 |
GSM3893106 |
Leaves, Man-12h-3 |
GSM3893107 |
Leaves, Man-24h-1 |
GSM3893108 |
Leaves, Man-24h-2 |
GSM3893109 |
Leaves, Man-24h-3 |
GSM3893110 |
Leaves, Man-72h-1 |
GSM3893111 |
Leaves, Man-72h-2 |
GSM3893112 |
Leaves, Man-72h-3 |
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Relations |
SRA |
SRP187794 |
Supplementary file |
Size |
Download |
File type/resource |
GSE132824_Abiotic_RNA-Seq.Readcount.txt.gz |
2.3 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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