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Series GSE132738 Query DataSets for GSE132738
Status Public on Jun 24, 2019
Title A Stromal Cell Niche Sustains ILC2-Mediated Type-2 Conditioning in Adipose Tissue
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Group-2 innate lymphoid cells (ILC2), type-2 cytokines and eosinophils, have all been implicated in sustaining adipose tissue homeostasis. However, the interplay between the stroma and adipose-resident immune cells is less well understood. We identify that white adipose tissue-resident multipotent stromal cells (WAT-MSCs) can act as a reservoir for IL-33, especially after cell stress, but also provide additional signals for sustaining ILC2. Indeed, we demonstrate that WAT-MSCs also support ICAM-1-mediated proliferation and activation of LFA-1-expressing ILC2s. Consequently, ILC2-derived IL-4 and IL-13 feed-back to induce eotaxin secretion from WAT-MSCs supporting eosinophil recruitment. Thus, MSCs provide a niche for multifaceted dialogue with ILC2 to sustain a type-2 immune environment in WAT.
Overall design White adipose tissue from IL-33-critine heterozygous reporter mice was mechanically dissociated in RPMI-1640, and digested with collagenase I (Life Technologies), DNase I (Roche) at 37oC whilst shaking. Lin-CD45-Epcam-CD31-CD24-PDGFRα+CD34+Sca1+Podoplanin+ IL-33-citrine+ or IL-33-citrine- WAT-MSCs, Lin-CD45-Epcam+ and Lin-CD45-Epcam-CD31+ were sorted by flow cytometry using Sony iCyt Synergy (Sony) to >95% purity. Three replicates from independent experiments were obtained and sequenced for RNA-seq.
Contributor(s) Rana BM, Jou E, Barlow JL, Rodriguez-Rodriguez N, Walker JA, Knox C, Jolin HE, Hardman CS, Sivasubramaniam M, Stezo A, Cohen ES, Scott IC, Sleeman MA, Chidomere CI, Cruz Migoni S, Caamano J, Jorgensen HF, Carobbio S, Vidal-Puig A, McKenzie AN
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Submission date Jun 14, 2019
Last update date Jun 24, 2019
Contact name Noe Rodriguez Rodriguez
Organization name MRC Laboratory of Molecular Biology
Department PNAC
Lab Andrew McKenzie
Street address Cambridge Biomedical Campus, Francis Crick Ave
City Cambridge
State/province Cambridgeshire
ZIP/Postal code CB2 0QH
Country United Kingdom
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (15)
GSM3891011 Lung Epcam+ epithelial cells Replicate 1
GSM3891012 Lung Epcam+ epithelial cells Replicate 2
GSM3891013 Lung Epcam+ epithelial cells Replicate 3
BioProject PRJNA548874
SRA SRP201434

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Supplementary file Size Download File type/resource
GSE132738_IL33_MSC_Epi_Endo.txt.gz 457.3 Kb (ftp)(http) TXT
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Processed data are available on Series record

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