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Series GSE132641 Query DataSets for GSE132641
Status Public on Jun 26, 2019
Title Dramatic changes in gene expression in adherent C. fasciculata reveal potential mechanisms for insect-specific adhesion of kinetoplastid parasites
Organisms Crithidia fasciculata; Aedes aegypti
Experiment type Expression profiling by high throughput sequencing
Summary Kinetoplastids are a group of parasite species, several of which cause important diseases in human and livestock. Nearly all of these pathogenic species are transmitted by insect vectors, in which the parasites undergo a specific developmental program. One shared event undergone by multiple species is adherence to insect tissue. This adhesion occurs by means of a hemidesmosome-like structure that is thus far uncharacterized. We have used the monoxenous parasite Crithidia fasciculata, which exclusively infects mosquitoes, to study this process of parasite adhesion in the insect. We have transcriptionally profiled adherent and swimming forms of the parasite that have been generated in vitro, and compared these profiles to the adhesive form in the mosquito. Using a dual-RNAseq approach, we have also identified several genes that are differentially regulated in infected versus uninfected mosquitoes, including several immune genes. This indicates that the mosquito is responding to the presence of the parasites.
Overall design We isolated RNA from three flasks containing exclusively swimming C. fasciculata, and from three flasks containing exclusively adherent C. fasciculata. One of the replicates of adherent cells was excluded for technical reasons. We also infected three separate generations of mosquitoes with C. fasciculata, isolated RNA, and compared them RNA from age-matched uninfected controls.
Contributor(s) Filosa J, Berry CT, Ruthel G, Beverley SM, Warren WC, Tomlinson C, Myler P, Dudkin E, Povelones ML, Povelones M
Citation(s) 31356610
Submission date Jun 12, 2019
Last update date Aug 21, 2019
Contact name Megan Povelones
Organization name Penn State Brandywine
Department Sciences
Street address 25 Yearsley Mill Road
City Media
State/province PA
ZIP/Postal code 19063
Country USA
Platforms (3)
GPL22030 Illumina NextSeq 500 (Aedes aegypti)
GPL26772 Illumina NextSeq 500 (Crithidia fasciculata)
GPL26774 Illumina NextSeq 500 (Aedes aegypti; Crithidia fasciculata)
Samples (12)
GSM3884602 Swimming replicate 1
GSM3884603 Swimming replicate 2
GSM3884604 Swimming replicate 3
BioProject PRJNA548563
SRA SRP201250

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Supplementary file Size Download File type/resource
GSE132641_Aedes_EstCounts.csv.gz 602.9 Kb (ftp)(http) CSV
GSE132641_Aedes_NormData_ComBat.csv.gz 422.8 Kb (ftp)(http) CSV
GSE132641_CfCultured_Norm.csv.gz 340.6 Kb (ftp)(http) CSV
GSE132641_CfCulturedandHindgut_EstCounts.csv.gz 334.2 Kb (ftp)(http) CSV
GSE132641_CfCulturedandHindgut_Norm.csv.gz 434.6 Kb (ftp)(http) CSV
GSE132641_TriTrypDB-28_CfasciculataCfCl_AnnotatedTranscripts.fasta.gz 5.5 Mb (ftp)(http) FASTA
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Raw data are available in SRA
Processed data are available on Series record

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