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Series GSE132598 Query DataSets for GSE132598
Status Public on Feb 03, 2020
Title Gene expression profiles of control or PERK siRNA-transfected brown adipocyte during the differentiation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The area of mitochondria in differentiated brown adipocytes is dramatically increased compared with that in brown pre-adipocytes. The ATP production is switched from the glycolysis pathway to the OXPHOS pathway during brown adipocyte differentiation. Endoplasmic reticulum (ER) is surrounded by mitochondria and the area of contact sites between mitochondria and the ER is increased. ER stress sensor PERK was phosphorylated during differentiation. To elucidate the mitochondria-ER crosstalk signaling mediated by PERK, RNA-sequencing analysis was performed using control siRNA- or PERK siRNA-transfected brown adipocytes.
 
Overall design Examination of gene expression in control or PERK siRNA-transfected brown adipocyte during the differentiation.
 
Contributor(s) Nishitoh H, Kato H, Tanimoto K
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Submission date Jun 12, 2019
Last update date Feb 05, 2020
Contact name Hideki Nishitoh
E-mail(s) nishitoh@med.miyazaki-u.ac.jp
Organization name University of Miyazaki
Department Department of Medical Sciences
Lab Laboratory of Biochemistry and Molecular Biology
Street address 5200 Kihara, Kiyotake
City Miyazaki City
State/province Miyazaki Prefecture
ZIP/Postal code 889-1692
Country Japan
 
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (4)
GSM3878616 mouse_BAT_sicont16hr
GSM3878617 mouse_BAT_siPERK16hr
GSM3878618 mouse_BAT_sicont24hr
Relations
BioProject PRJNA548519
SRA SRP201230

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE132598_RAW.tar 3.1 Mb (http)(custom) TAR (of FPKM_TRACKING)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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