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| Status |
Public on Jun 12, 2019 |
| Title |
Single-cell omics reveal human mononuclear phagocyte heterogeneity and inflammatory DC in health and disease |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Human mononuclear phagocytes comprise phenotypically and functionally overlapping subsets of dendritic cells (DC) and monocytes, but their identification remains elusive. By integrating high-dimensional single-cell protein and RNA expression data, we clearly delineated monocytes from conventional DC2 (cDC2), identifying new markers including CD88/CD89 for monocytes and HLA-DQ/FcRI for cDC2, allowing their unambiguous characterization in blood and tissues. We also show that cDC2 can be subdivided into phenotypically and functionally distinct subsets based on CD5, CD163 and CD14 expression, including a unique subset of circulating inflammatory CD5-CD163+CD14+ cells related to previously defined DC3. These inflammatory DC3 were expanded in systemic lupus erythematosus patients, correlating with disease activity. Unravelling the heterogeneity of DC sub-populations in health and disease paves the way for specific DC subset-targeting therapies.
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| Overall design |
Indexed single cell RNAseq (scRNAseq) of human peripheral blood dendritic cells and monocytes
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| Web link |
https://doi.org/10.1016/j.immuni.2019.08.008
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| Contributor(s) |
Dutertre C, Becht E, Irac SE, Khalilnezhad A, Narang V, Khalilnezhad S, Ng PY, vandenHoogen LL, Leong JY, Lee B, Chevrier M, Zhang XM, Ai Yong PJ, Koh G, Lum J, Howland SW, Mok E, Chen J, Larbi A, Tan HK, Lim TH, Karagianni P, Tzioufas AG, Malleret B, Brody J, Albani S, van Roon J, Radstake T, Newell EW, Ginhoux F |
| Citation(s) |
31474513 |
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| Submission date |
Jun 11, 2019 |
| Last update date |
Sep 11, 2019 |
| Contact name |
Florent Ginhoux |
| E-mail(s) |
Florent_Ginhoux@immunol.a-star.edu.sg
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| Organization name |
Singapore Immunology Network (SIgN), A*STAR
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| Street address |
8A Biomedical Grove, Immunos Building, Level 3
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| City |
Singapore |
| ZIP/Postal code |
138648 |
| Country |
Singapore |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (221)
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| This SubSeries is part of SuperSeries: |
| GSE136732 |
Single-Cell Analysis of Human Mononuclear Phagocytes Reveals Subset-Defining Markers and Identifies Circulating Inflammatory Dendritic Cells |
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| Relations |
| BioProject |
PRJNA548370 |
| SRA |
SRP201124 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE132566_RAW.tar |
34.9 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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