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Series GSE132364 Query DataSets for GSE132364
Status Public on Jun 28, 2019
Title Single-cell transcriptomics of the embryonic mouse pancreas
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Data accompaning to van Gurp et al. Development 2019. single-cell sequencing of the developing mouse pancreas followed by Seurat analysis to discover genes important for alpha and beta cell differentiation.
 
Overall design Single-cells from mouse embryonic pancreas at E12.5, E13.5, E14.5, E15.5 and E18.5 were isolated and enriched for MIP-GFP and sorted into 384-well plates. Afterwards, SORT-seq was performed and single-cell transcriptomics profiles were obtained.
 
Contributor(s) van Gurp L, Muraro MJ, van Oudenaarden A, de Koning E
Citation(s) 31160419
Submission date Jun 07, 2019
Last update date Oct 01, 2019
Contact name Mauro Muraro
E-mail(s) m.muraro@scdiscoveries.com
Organization name Single Cell Discoveries
Street address Uppsalalaan, 8
City Utrecht
State/province Utrecht
ZIP/Postal code 3584 CT
Country Netherlands
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (58)
GSM3895638 Embryonic pancreas day E12.5, live population (Dapi -)
GSM3895639 Embryonic pancreas day E12.5, MIP-GFP population
GSM3895640 Embryonic pancreas day E15.5, live population (Dapi -)
Relations
BioProject PRJNA549972
SRA SRP202046

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE132364_monocle_subset.R.gz 625 b (ftp)(http) R
GSE132364_monocle_subset.Rdata.gz 31.3 Mb (ftp)(http) RDATA
GSE132364_rawData_full.txt.gz 34.4 Mb (ftp)(http) TXT
GSE132364_rawData_subset.txt.gz 14.8 Mb (ftp)(http) TXT
GSE132364_readme_demultiplexing_Cel-seq1_data.pdf 85.9 Kb (ftp)(http) PDF
GSE132364_readme_demultiplexing_Cel-seq2_data.pdf 811.8 Kb (ftp)(http) PDF
GSE132364_seurat_full.R.gz 922 b (ftp)(http) R
GSE132364_seurat_full.Rdata.gz 760.7 Mb (ftp)(http) RDATA
GSE132364_seurat_subset.R.gz 951 b (ftp)(http) R
GSE132364_seurat_subset.Rdata.gz 405.4 Mb (ftp)(http) RDATA
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Processed data are available on Series record
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