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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 17, 2019 |
| Title |
RNA Seq analyzed the transcriptional difference between the ICSI and ICAHCI embryo in blastocyst and placenta |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Our lab first derived mouse androgenetic haploid embryonic stem cells (AG-haESCs) and demonstrated that AG-haESCs can be used as an “artificial spermatids” to generate gene-edited semi-cloned (SC) mice through intracytoplasmic injection (ICAHCI) into mature oocyte, even though the birth efficiency is very low. Further we proved that H19-DMR and IG-DMR were the main barrier to generate viable mice through androgenetic and parthenogenetic haESCs. More importantly, AG-haESCs mediated SC technology combined with CRISPR-Cas9 is a powerful tool to generate gene-modified mouse models and carry out genetic screening at organismal level. However, it is still not clear how the H19-DMR and IG-DMR coordinately regulate SC embryo development. Here, we found that the H19-DMR and IG-DMR regulate the development of SC embryos in spatio-temporal scales. Firstly, we found that the H19-DMR and IG-DMR are not indispensable for the development of preimplantation of SC embryos. Secondly, H19-DMR is essential for the development of SC embryos in mid-gestation and IG-DMR takes effect in late-gestation. Further, the maintenance of paternal H19-DMR methylation status and deletion of paternal H19 transcription unit play a key role in the structures and transport functions of SC embryo placenta. Importantly, AG-haESCs carrying triple deletions, including H19, H19-DMR and IG-DMR, can further improve the efficiency in generation of viable, normal-size, and fertile mice.
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| Overall design |
For blastocyst, five samples (including ICM and TE) for control (ICSI) and semi-cloned blastocysts (including WT and DKO). For placenta, three samples for control (ICSI) and semi-cloned placentae (including WT, DKO, IG△DMR and H19△13kb)
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| Contributor(s) |
Li J, Li Q, Li Y |
| Citation(s) |
31564034 |
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| Submission date |
Jun 05, 2019 |
| Last update date |
Oct 13, 2019 |
| Contact name |
Qing Li |
| E-mail(s) |
liqing2015@sibcb.ac.cn
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| Organization name |
Chinese Academy of Science
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| Department |
institute of biochemistry and cell biology
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| Lab |
Jinsong Li's Lab
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| Street address |
320 Yueyang Road
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| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (2) |
| GPL21273 |
HiSeq X Ten (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (45)
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| Relations |
| BioProject |
PRJNA546605 |
| SRA |
SRP200552 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE132254_RAW.tar |
6.9 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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