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| Status |
Public on Jun 06, 2019 |
| Title |
Comparative transcriptomics between species to assess the immune mechanisms underlying the increased reactogenicity of the four component meningococcal capsular group B vaccine when given concomitantly with routinely-administered vaccines |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Whole blood RNA-seq and single cell RNA-seq on neutrophils was leveraged to explore gene expression changes induced in mice 24 hours after immunisation with a second dose of a licensed vaccine against capsular group B, meningococcus, the same vaccine in combination with routinely-administered vaccines, routine vaccines on their own, or a PBS control immunisation.
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| Overall design |
mRNA was profiled from RNA extracted from mouse neutrophils or whole blood, 6 samples per group (neutrophils pool from each sample to yield one sample per group for single cell sequencing) , using an Illumina HiSeq4000 or NovaSeq, respectively.
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| Contributor(s) |
Sheerin D |
| Citation(s) |
31551511 |
| |
| Submission date |
Jun 05, 2019 |
| Last update date |
Oct 07, 2019 |
| Contact name |
Dylan Sheerin |
| E-mail(s) |
dylan.sheerin@paediatrics.ox.ac.uk
|
| Organization name |
University of Oxford
|
| Department |
Paediatrics
|
| Lab |
Oxford Vaccine Group
|
| Street address |
Centre for Clinical Vaccinology and Tropical Medicine, Churchill Hospital
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| City |
Oxford |
| ZIP/Postal code |
OX37LJ |
| Country |
United Kingdom |
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| Platforms (2) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA546495 |
| SRA |
SRP200453 |
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