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Series GSE132188 Query DataSets for GSE132188
Status Public on Jun 11, 2019
Title Comprehensive single-cell mRNA profiling reveals a detailed roadmap for pancreatic endocrinogenesis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary This dataset consists of single-cell RNA-seq (10X) data from 4 embryonic stages (E12.5-15.5) of pancreatic epithelial cells from Neurogenin3 (Ngn3)-Venus fusion (NVF) homozygous mice. Endocrine progenitor cells (NVF+) were enriched by FACS cell sorting.
 
Overall design 4 single cell RNA seq experiments were performed using 10X Genomics technology to generate single cell transcriptomic profiles of embryonic pancreatic epithelial cells from a Neurogenin3 (Ngn3)-Venus fusion (NVF) reporter mouse line. Cells were sorted and NVF+ and NVF- cells mixed (50:50) to enrich for endocrine Ngn3+ progenitors. Cells were sampled from pancreata of E12.5,E13.5,E14.5 and E15.5 embryos.
 
Contributor(s) Bastidas-Ponce A, Tritschler S, Leander D, Scheibner K, Tarquis-Medina M, Salinno C, Schirge S, Burtscher I, Böttcher A, Theis FJ, Lickert H, Bakhti M
Citation(s) 31160421
Submission date Jun 04, 2019
Last update date Sep 10, 2019
Contact name Sophie Tritschler
E-mail(s) sophie.tritschler@helmholtz-muenchen.de
Organization name Helmholtz Zentrum Munich
Street address Ingolstädter Landstrasse 1
City Neuherberg
ZIP/Postal code 85764
Country Germany
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (4)
GSM3852752 E12_5
GSM3852753 E13_5
GSM3852754 E14_5
Relations
BioProject PRJNA546282
SRA SRP200419

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE132188_RAW.tar 410.5 Mb (http)(custom) TAR (of TAR)
GSE132188_adata.h5ad.h5 318.2 Mb (ftp)(http) H5
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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