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| Status |
Public on Oct 01, 2019 |
| Title |
Dual modulation of neuron specific microRNAs and the REST complex promotes functional maturation of induced human adult neurons |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Direct neural reprogramming can be achieved using different approaches, including by expressing neuronal transcription factors or microRNAs, and by knocking down neuronal repressive elements. However, there still exists a high variability in terms of the quality and maturity of the induced neurons (iNs) obtained, depending on the reprogramming strategy employed. Here, we evaluate different long-term culture conditions and study the effect of expressing the neuronal-specific microRNAs miR124 and miR9/9* while reprogramming with Ascl1, Brn2 and knockdown of the neuronal repressor REST. We show that addition of microRNA supports neuronal maturation both in terms of gene and protein expression, as well as promotes the development of active ion channels and the ability of iNs to generate current induced and spontaneous action potentials.
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| Overall design |
RNA sequencing of induced human neurons from fibroblasts with two different conditions. Three replicatess were used for each condition.
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| Contributor(s) |
Sharma Y |
| Citation(s) |
31535361 |
| |
| Submission date |
Jun 04, 2019 |
| Last update date |
Jan 09, 2020 |
| Contact name |
Yogita Sharma |
| E-mail(s) |
yogita.sharma@med.lu.se
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| Organization name |
Lund University
|
| Street address |
Solvegatan 17
|
| City |
Lund |
| ZIP/Postal code |
22184 |
| Country |
Sweden |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA546243 |
| SRA |
SRP200390 |
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