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Series GSE131996 Query DataSets for GSE131996
Status Public on Jun 25, 2019
Title Neuropeptide CGRP limits ILC2 responses and constrains type 2 inflammation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Innate and adaptive lymphocytes work in concert to maintain tissue homeostasis and to mediate host defense at mucosal barriers. Herein, we used single cell analysis to show substantial diversity of gene expression in ILCs and T helper cells during a helminth infection in the lung. Notably, we found that the Calca gene, which is spliced to generate the neuropeptide CGRP, was selectively transcribed in ILC2s and Th2 cells in an activation dependent manner. The Calca locus acquired chromatin accessibility at the ILC2 precursor stage and is pre-programmed for rapid production of CGRP upon ILC2 activation. CGRP globally antagonized actions of neuromedin U (NMU) and the alarmin IL-33. However, CGRP selectively acted in concert with NMU and IL-33 to promote IL-5 expression, but not IL-13. The complex interplay among neuropeptides and alarmin fine-tunes type 2 immune responses and will undoubtedly become more relevant as therapeutic neuropeptide blockade advances in the clinic.
Overall design Integrated analysis of transcriptome and epigenome data from ILCs and Th cells in steady or activated conditions
Contributor(s) Nagashima H, Shih HY, Sun HW, Brooks SR, Gutierrez-Cruz G, Dell'Orso S, Kanno Y, O'Shea JJ
Citation(s) 31353223
Submission date May 30, 2019
Last update date Nov 28, 2019
Contact name Yuka Kanno
Phone 301-402-3008
Organization name NIH
Department NIAMS
Street address 10 Center Drive, Rm13C120
City Bethesda
State/province MD
ZIP/Postal code 20892-1930
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (54)
GSM3834103 FastATAC_ILC2_nostim_4h_rep1
GSM3834104 FastATAC_ILC2_nostim_4h_rep2
GSM3834105 FastATAC_ILC2_CGRP_4h_rep1
BioProject PRJNA545529
SRA SRP199932

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Supplementary file Size Download File type/resource
GSE131996_RAW.tar 8.5 Gb (http)(custom) TAR (of BW, MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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