|
| Status |
Public on Feb 14, 2020 |
| Title |
Paired ChIP-Seq studies of Kasumi-1 t(8;21) AML cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Nearly 10-15% of all acute myeloid leukemia (AML) cases are caused by a recurring chromosomal translocation between 8 and 21, t(8;21). The t(8;21) translocation generates the AML1-ETO leukemia fusion protein. AML1-ETO promotes leukemogenesis by transcriptionally dysregulating important cell-fate genes. Here, to better understand how AML1-ETO deregulates transcription, we performed paired ChIP-Seq analyses of sequence-specific transcription factors, coactivators, corepressors, HDACs, RNA Pol II and acetyl-histone marks in both control and AML1-ETO-depleted Kasumi-1 t(8;21) AML cells.
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| |
|
| Overall design |
Chromatin immunoprecipitation using specific antibodies followed by deep sequencing in Kasumi-1 t(8;21) AML cells.
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| |
|
| Contributor(s) |
Zhang J |
| Citation(s) |
32071087 |
| |
| Submission date |
May 29, 2019 |
| Last update date |
May 15, 2020 |
| Contact name |
Jinsong Zhang |
| E-mail(s) |
jinsongzhang@slu.edu
|
| Phone |
(314)977-6496
|
| Organization name |
Saint Louis University School of Medicine
|
| Department |
Department of Pharmacology & Physiology
|
| Street address |
1402 South Grand Blvd.
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63104 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (26)
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|
| Relations |
| BioProject |
PRJNA545353 |
| SRA |
SRP199800 |
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