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Series GSE131869 Query DataSets for GSE131869
Status Public on Nov 25, 2019
Title Cx3cr1-deficient microglia exhibit a premature aging transcriptome
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary CX3CR1, one of the highest expressed genes in microglia in mice and humans, is implicated in numerous microglial functions. However, the molecular mechanisms underlying Cx3cr1 signaling are not well understood. Here, we analyzed transcriptomes of Cx3cr1-deficient microglia under varying conditions by RNA sequencing (RNA-Seq). In 2 mos mice, Cx3cr1 deletion resulted in the downregulation of a subset of immune-related genes, without substantial epigenetic changes in markers of active chromatin. Surprisingly, Cx3cr1-deficient microglia from young mice exhibited a transcriptome consistent with that of aged Cx3cr1-sufficient animals, suggesting a premature aging transcriptomic signature.
Overall design RNAseq analysis of sorted microglia
Contributor(s) Gyoneva S, Hosur R, Challa R, Cullen P, Miao K, Allaire N, Roberts C
Citation(s) 31792059
Submission date May 28, 2019
Last update date Dec 31, 2019
Contact name Stefka Gyoneva
Phone 617-914-6196
Organization name Biogen, Inc
Street address 225 Binney St.
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (155)
GSM3823683 2mo-WT-1M
GSM3823684 2mo-WT-2M
GSM3823685 2mo-WT-3M
BioProject PRJNA545148
SRA SRP199678

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE131869_CX3CR1_LPS_genes.estcount_table.txt.gz 2.5 Mb (ftp)(http) TXT
GSE131869_CX3CR1_LPS_genes.fpkm_table.txt.gz 2.5 Mb (ftp)(http) TXT
GSE131869_CX3CR1_LPS_genes.tpm_table.txt.gz 2.5 Mb (ftp)(http) TXT
GSE131869_CX3CR1_aging_genes.estcount_table.txt.gz 3.7 Mb (ftp)(http) TXT
GSE131869_CX3CR1_aging_genes.fpkm_table.txt.gz 3.6 Mb (ftp)(http) TXT
GSE131869_CX3CR1_aging_genes.tpm_table.txt.gz 3.8 Mb (ftp)(http) TXT
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