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Series GSE131772 Query DataSets for GSE131772
Status Public on Aug 08, 2019
Title Genome-wide occupancy of BDR1 and BDR3 (ChIP-seq)
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We generated Arabidopsis lines expressing tagged (3X Myc tag) versions of BDR1 or BDR3 driven by their endogenous promoters on a bdrs triple mutant background (bdrs: cross of bdr1/AT5G25520 mutant SALK_142108C, bdr2/AT5G11430 mutant CS852350 and bdr3/AT2G25640 mutant SALK_059905). The genome-wide distribution of BDR1 and BDR3 in 8-day old seedlings were analyzed by ChIP-seq using an anti Myc antibody. As control, we performed in parallel a ChIP in wild-type seedlings using the anti-Myc tag antibody. We found that BDR1 is enriched at the border of a large number of genes in Arabidopsis genome while BDR3 is mostly enriched at the 3'-end of genes.
Overall design ChIP-seq profiles in 8-day old seedlings of Myc tag-BDR1 and Myc tag-BDR3 (Myc tag-BDR proteins expressed in bdrs triple mutant background). Control is a ChIP perfomed in wild-type plants (not expressing any tagged protein) with the anti-Myc tag antibody.
Contributor(s) Michaels SD, Martin PG, Yu X, Huang J, Liu J
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Submission date May 25, 2019
Last update date Aug 10, 2019
Contact name Pascal GP Martin
Organization name INRA
Lab ToxAlim
Street address 180 chemin de Tournefeuille, BP 93173
City Toulouse Cedex 3
ZIP/Postal code 31027
Country France
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (4)
GSM3819643 Myc WT
GSM3819644 Myc BDR1
GSM3819645 Myc BDR3 rep1
This SubSeries is part of SuperSeries:
GSE112443 RNA-seq, ChIP-seq and MNase-seq in WT, fpa mutant and bdr1,2,3 mutant Arabidopsis seedlings
BioProject PRJNA544799
SRA SRP199505

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE131772_RAW.tar 934.9 Mb (http)(custom) TAR (of BED, BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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