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| Status |
Public on Aug 08, 2019 |
| Title |
Dynamic Lineage Priming by ERK is Driven by Transcription Factor-Independent Enhancer Regulation [gene expression] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Extensive analysis if the means by which ERK affects transcription via enhancer regulation in mouse embryonic stem cells. This study combines analysis of mRNA, and nascent RNA dynamics and correlates this with changes in transcription factor, co-factor, and RNAPII binding and activity.
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| |
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| Overall design |
WT or KO ESCs were stimulated for 8hrs, or stimulated for 8hrs with 4OHT to activate the Raf-ErT2 fusion they express and then with a subesquent 2hr treatment with the Mek inbhibitor PD03
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| |
|
| Contributor(s) |
Hamilton WB, Brickman JM |
| Citation(s) |
31695196 |
| |
| Submission date |
May 21, 2019 |
| Last update date |
Nov 11, 2019 |
| Contact name |
Josh M Brickman |
| E-mail(s) |
joshua.brickman@sund.ku.dk
|
| Organization name |
Copenhagen University
|
| Department |
reNEW
|
| Lab |
Brickman
|
| Street address |
3B Blegdamsvej
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platforms (1) |
| GPL21810 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Feature Number version] |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE132444 |
Dynamic Lineage Priming by ERK is Driven by Transcription Factor-Independent Enhancer Regulation |
|
| Relations |
| BioProject |
PRJNA544001 |
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