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Series GSE131525 Query DataSets for GSE131525
Status Public on Feb 03, 2020
Title A composite immune signature parallels disease progression across T1D subjects (RNA-Seq Cohort 0 Cell Sorted)
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Technical replicate testing was performed to determine the measurement precision of each analyte. Peripheral blood mononuculear cells were processed and frozen down from blood samples collected from healthy control subjects (n=3) or subjects with Systemic Lupus Erythematosus (n=3). Three separate PBMC aliquots per subject were then independently thawed and sorted by flow into B cell, CD4+ T cell, CD8+ T cell, and monocyte populations. Total RNA was isolated from the sorted cell populations and then RNAseq libraries were prepared.
Overall design The replicate testing cohort was used to determine the technical precision of each analyte by calculating the intra-subject coefficient of variation (CV) for three replicate aliquots (n=3) per blood draw from 6 subjects. For each analyte, the mean CV across subjects was then calculated. Three independent PBMC aliquots per subject were thawed and sorted by flow cytometry into B cell, CD4+ and CD8+ T cell, and monocyte subsets before RNA sequencing.
Contributor(s) Speake C, Skinner SO, Berel D, Whalen E, Dufort MJ, Odegard JM, Pesenacker AM, Gorus FK, James EA, Levings MK, Linsley PS, Akirav EM, Pugliese A, Hessner MJ, Nepom GT, Gottardo R, Long SA
Citation(s) 31671072
Submission date May 20, 2019
Last update date Jul 28, 2021
Contact name Scott Presnell
Organization name Benaroya Research Institute
Department Systems Immunology
Street address 1201 Ninth Ave.
City Seattle
State/province WA
ZIP/Postal code 98101
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (72)
GSM3785265 lib3876
GSM3785266 lib3877
GSM3785267 lib3878
This SubSeries is part of SuperSeries:
GSE131528 A composite immune signature parallels disease progression across T1D subjects
BioProject PRJNA543894
SRA SRP199000

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Supplementary file Size Download File type/resource
GSE131525_CAV_Replicate_testing_RNAseq_cell_subset_combined_counts.csv.gz 2.0 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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