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| Status |
Public on May 16, 2022 |
| Title |
Constitutive activity of the cation channel TRPM8 regulates the function and differentiation of human monocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This study investigated the impact of TRPM8 activity on human monocyte differentiation and function. CD14+ peripheral blood monocytes were isolated from healthy volunteers and differentiated into macrophages using M-CSF, in the presence or absence of 10μM AMTB (TRPM8 antagonist). RNA seq was performed on CD14+ monocytes and macrophages differentiated in vehicle(Mo-M-DMSO) or AMTB (Mo-M-AMTB). To assess the transcriptional changes that normally occur in monocyte to macrophage differentiaiton we compared the Mo-M-DMSO and CD14+ monocyte samples. 3109 genes were significantly down-regulated and 3403 up-regulated in Mo-M-DMSO samples compared to CD14+ monocytes. To investigate the impact of AMTB upon macrophage differentiation we compared the Mo-M-DMSO and Mo-M-AMTB. 556 genes were significantly lower and 421 genes were significantly higher in Mo-M-AMTB samples compared to Mo-M-DMSO samples. 331 out of the 556 genes lower in Mo-M-AMTB samples were genes that were normally up-regulated during differentiaiton and 253 out of the 421 genes higher in the Mo-M-AMTB samples were genes that were normally down-regulated during differentiation indicating that AMTB predominantly acts to inhibit aspects of the differentiation programme.
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| Overall design |
Peripheral blood CD14+ monocytes from 5 healthy donors were differentiated into macrophages (Mo-M) by 6 days culture with M-CSF, in the presence of 10μM AMTB or vehicle (DMSO). PolyA+ RNA seq was performed on CD14+ monocytes, Mo-M-DMSO and Mo-M-AMTB samples.
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| Contributor(s) |
Hornsby E, Stagg AJ, King HW |
| Citation(s) |
35233801 |
| |
| Submission date |
May 17, 2019 |
| Last update date |
Oct 28, 2022 |
| Contact name |
Hamish W King |
| Organization name |
Queen Mary University of London
|
| Department |
Blizard Institute
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| Street address |
4 Newark St
|
| City |
London |
| ZIP/Postal code |
E1 2AT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA543501 |
| SRA |
SRP198780 |
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