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| Status |
Public on Apr 21, 2020 |
| Title |
m6A-Label-seq directly reports m6A methylome at base resolution |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
Other
Expression profiling by high throughput sequencing
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| Summary |
Here we report a metabolic labeling method to map mRNA N6-methyladenosine (m6A) modification transcriptome-wide at base resolution, termed m6A-label-seq. The cells were fed with Se-allyl-L-selenohomocysteine, an analog of methoine, which serves as the precursor of methylation enzyme cofactor, so that cellular RNAs were continuously deposited with N6-allyladenosine (a6A) at supposed m6A sites. We enriched a6A-containing mRNAs and sequenced their a6A sites which are identical to m6A sites, based on iodination-induced misincorporation during reverse transcription.
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| Overall design |
We profiled m6A sites at base resolution in human HeLa and HEK293T cells and mouse H2.35 cells by metabolically incorporation of Se-derived methionine analog.
We assessed the potential stress response in HeLa cells by an RNA-seq experiment.
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| Contributor(s) |
Cao J |
| Citation(s) |
32341503 |
| |
| Submission date |
May 16, 2019 |
| Last update date |
Jul 22, 2020 |
| Contact name |
Jie Cao |
| E-mail(s) |
cao_jie@zju.edu.cn
|
| Organization name |
Zhejiang University
|
| Department |
Department of Polymer Science and Engineering
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| Street address |
Zheda Road 38
|
| City |
Hangzhou |
| ZIP/Postal code |
310027 |
| Country |
China |
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| Platforms (2) |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA543237 |
| SRA |
SRP198610 |
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