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Series GSE130960 Query DataSets for GSE130960
Status Public on Oct 01, 2019
Title Cx3cr1-deficient microglia exhibit a premature aging transcriptome
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary CX3CR1, one of the highest expressed genes in microglia in mice and humans, is implicated in numerous microglial functions. However, the molecular mechanisms underlying Cx3cr1 signaling are not well understood. Here, we analyzed transcriptomes of Cx3cr1-deficient microglia under varying conditions by RNA-Seq. In 2 mos mice, Cx3cr1 deletion resulted in the downregulation of a subset of immune-related genes, without substantial epigenetic changes in markers of active chromatin. Surprisingly, Cx3cr1-deficient microglia from young mice exhibited a transcriptome consistent with that of aged Cx3cr1-sufficient animals, suggesting a premature aging transcriptomic signature. Immunohistochemical analysis of microglia in young and aged mice revealed that loss of Cx3cr1 modulates microglial morphology in a compatible fashion. Our results suggest that CX3CR1 may regulate microglial function in part by modulating the expression levels of a subset of inflammatory genes during chronological aging, making Cx3cr1-deficient mice useful for studying aged microglia.
Overall design H3K27ac and Rpb2 ChIP-seq for microglia C57BL/6J mice and Cx3cr1-deficient mice.
Contributor(s) Gyoneva S, Hosur R, Gosselin D, Zhang B, Ouyang Z, Cotleur AC, Peterson M, Allaire N, Challa R, Cullen P, Roberts C, Miao K, Reynolds TL, Glass CK, Burkly L, Ransohoff RM
Citation(s) 31792059
Submission date May 09, 2019
Last update date Dec 31, 2019
Contact name Christopher K Glass
Organization name University of California, San Diego
Department School of Medicine
Street address 9500 Gilman Drive
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (12)
GSM3757228 Cx3cr1KO_H3k27ac_F
GSM3757229 Cx3cr1KO_H3k27ac_M
GSM3757230 Cx3cr1KO_Input_F
BioProject PRJNA542087
SRA SRP197306

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE130960_H3k27ac.peak.tag.txt.gz 5.1 Mb (ftp)(http) TXT
GSE130960_Rbp2.peak.tag.txt.gz 2.5 Mb (ftp)(http) TXT
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Processed data are available on Series record

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