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| Status |
Public on Jul 09, 2019 |
| Title |
Stem cell-derived cranial and spinal motor neurons reveal proteostatic differences between ALS resistant and sensitive motor neurons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the comparative gene expression between embryonic stem cell derived cranial and spinal motor neurons and multiple time points after induction and primary cultured ocular and spinal motor neurons, using single cell RNA sequencing.
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| Overall design |
Single neurons were isolated in 96-well plates and their gene expression profiled using SMART-Seq2 from 8 samples: (1-2) primary cultured oculomotor/trochlear motor neurons and spinal motor neurons collected at embryonic day E11.5 and cultured for 7 days, (3-8) ESC-derived induced cranial and spinal motor neurons at either 2 days, 5 days, or 7 days after plating.
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| Contributor(s) |
Rose MF, An D, Fujiki R, Mazzoni EO, Engle EC |
| Citation(s) |
31157617 |
| |
| Submission date |
May 08, 2019 |
| Last update date |
Jul 09, 2019 |
| Contact name |
Matthew F Rose |
| E-mail(s) |
matthew.rose@childrens.harvard.edu
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| Organization name |
Boston Children's Hospital
|
| Department |
Neurology/Pathology
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| Lab |
Engle Laboratory
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| Street address |
3 Blackfan Circle
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (576)
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| Relations |
| BioProject |
PRJNA543533 |
| SRA |
SRP198810 |
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