GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE130566 Query DataSets for GSE130566
Status Public on Aug 27, 2019
Title Single-cell transcriptomic analysis of the scrib mutant wing imaginal discs
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary Investigation of intratumor heterogeneity in the scrib¹ mutant wing imaginal discs.
Method: Staged scrib¹ wing imaginal discs were dissected and transferred to DPBS. The wing imaginal discs were dissociated in 0.25% Trypsin-EDTA solution at 37 ℃ for 10 min. Cells were then washed in DPBS and passed through 35μm filter before library preparation. Construction of 10x single cell libraries and sequencing on Illumina platform were performed by Novogene.
Overall design 10X based single-cell mRNA profiles of 4, 5, 8, 14-day scrib mutant wing imaginal discs from prolonged larval stages.
Contributor(s) Zhang L, Ying W, Deng M, Yan Y
Citation(s) 31371383, 31455604
Submission date May 01, 2019
Last update date Dec 03, 2019
Contact name Yan Yan
Organization name HKUST
Department LIFS
Street address Clear Water Bay
City Hong Kong
ZIP/Postal code 999077
Country China
Platforms (1)
GPL25244 Illumina NovaSeq 6000 (Drosophila melanogaster)
Samples (4)
GSM3743635 Scrib_4d
GSM3743636 Scrib_5d
GSM3743637 Scrib_8d
BioProject PRJNA540653
SRA SRP194385

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE130566_RAW.tar 331.7 Mb (http)(custom) TAR (of CSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap