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Series GSE130513 Query DataSets for GSE130513
Status Public on Jul 25, 2019
Title Modeling the MYC-driven normal-to-tumour switch in breast cancer.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The potent MYC oncoprotein is deregulated in many human cancers, including breast carcinoma, and is associated with aggressive disease. To understand the mechanisms and vulnerabilities of MYC-driven breast cancer, we have generated an in vivo model that mimics human disease in response to MYC deregulation. MCF10A cells ectopically expressing a common breast cancer mutation in the PI3 kinase pathway (PIK3CAH1047R) lead to the development of organized acinar structures in mice. However, expressing both PIK3CAH1047R and deregulated-MYC lead to the development of invasive ductal carcinoma, thus creating a model in which a MYC-dependent normal-to-tumour switch occurs in vivo. These MYC-driven tumours exhibit classic hallmarks of human breast cancer at both the pathological and molecular levels. Moreover, tumour growth is dependent upon sustained deregulated MYC expression, further demonstrating addiction to this potent oncogene and regulator of gene transcription. We therefore provide a MYC-dependent model of breast cancer which can be assayed for in vivo tumour initiation, proliferation, and transformation from normal breast acini into invasive breast carcinoma. Taken together, we anticipate that this novel MYC-driven transformation model will be a useful research tool to both better understand MYC’s oncogenic function and identify therapeutic vulnerabilities.
Overall design RNA-seq was conducted on 3 biological replicates of each 10A.PE and 10A.PM xenograft tumours.
Contributor(s) Lourenco C, Kalkat M, Houlahan KE, De Melo J, Longo J, Done SJ, Boutros PC, Penn LZ
Citation(s) 31350286
Submission date Apr 30, 2019
Last update date Aug 21, 2019
Contact name Corey Lourenco
Organization name University of Toronto
Department Medical Biophysics
Lab Penn
Street address 101 College St., 13-601
City Toronto
State/province ON
ZIP/Postal code M5G 1L7
Country Canada
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (6)
GSM3741589 n1_10A.PE_1NT
GSM3741590 n2_10A.PE_3NT
GSM3741591 n3_10A.PE_4LT
BioProject PRJNA540502
SRA SRP194282

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE130513_DEA_10Axeno.csv.gz 451.9 Kb (ftp)(http) CSV
GSE130513_RAW.tar 2.2 Mb (http)(custom) TAR (of TAB)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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