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| Status |
Public on May 07, 2020 |
| Title |
ChIP-seq from Human U2OS Cell for CRY2 and RUVBL2 with DMSO or Cordycepin Treatment |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
To evaluate the effects of cordycepin on CRY2 and RUVBL2 binding on the chromatin sites, non-synchronized U2OS cells were treated with cordycepin (100 uM) for 1 h, then performed ChIP-seq. We found that cordycepin relieves CRY2 and RUVBL2 proteins from the chromatin for more than 60-70%.
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| |
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| Overall design |
To evaluate the occupancies of RUVBL2 and CRY2, when treated or untreated U2OS cells with cordycepin.
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| Contributor(s) |
Ju D, Wang J, Zhang EE |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 30, 2019 |
| Last update date |
May 09, 2020 |
| Contact name |
wang jiawen |
| E-mail(s) |
wangjiawen2013@gmail.com
|
| Organization name |
National institute of biological sciences
|
| Street address |
7 Science Park Road ZGC Life Science Park
|
| City |
Beijing |
| ZIP/Postal code |
102206 |
| Country |
China |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE130508 |
ChIP_seq for Bmal1 and Ruvbl2 in Mouse Liver and for BMAL1, RUVBL2, CRY1 and CRY2 in Human U2OS Cells |
|
| Relations |
| BioProject |
PRJNA540499 |
| SRA |
SRP194279 |
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