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Series GSE13040 Query DataSets for GSE13040
Status Public on Dec 30, 2008
Title E11.5 Mouse Endodermal Organ Gene Expression
Organism Mus musculus
Experiment type Expression profiling by array
Summary To study gene expression during endodermal organogenesis, we sought to identify genes expressed in restricted domains during organogenesis. For gene expression analysis, six morphologically distinct endodermal domains were dissected at E11.5: the esophageal region; the lung and distal tracheal region; the stomach region; the hepatic region; the dorsal and ventral pancreatic region; and the intestinal region. Through flow cytometric separation using EpCAM expression to distinguish endoderm from surrounding mesenchyme, pure populations of endoderm progenitors from the esophageal, lung, stomach, pancreatic, and intestinal regions were isolated. Expression of Liv2 was used to isolate a pure population of hepatic endoderm progenitors.

Keywords: cell type comparison
 
Overall design Three biological replicates each containing dissected organ domains from 10-12 pooled embryos flow cytometrically sorted to isolate endoderm were amplified using Ambion Illumina TotalPrep RNA Amplification kit and arrayed on Illumina MouseRef8 v2 chips
 
Contributor(s) Sherwood R
Citation(s) 19097184
Submission date Oct 03, 2008
Last update date Jun 14, 2018
Contact name Richard Sherwood
E-mail rsherw@fas.harvard.edu
Organization name Harvard University
Department Department of Molecular and Cellular Biology
Lab Douglas Melton
Street address 7 Divinity Ave
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (28)
GSM326633 E8.25 4-6s definitive endoderm 1
GSM326634 E8.25 4-6s definitive endoderm 2
GSM326635 E8.25 4-6s definitive endoderm 3
Relations
BioProject PRJNA109713

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13040_RAW.tar 3.1 Mb (http)(custom) TAR
GSE13040_nonorm_nobkgd.txt 19.6 Mb (ftp)(http) TXT
Raw data is available on Series record
Processed data included within Sample table

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