GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE130398 Query DataSets for GSE130398
Status Public on Jul 21, 2019
Title Heterozygous p53-R280T mutation promotes proliferation of NPC cells through activating PI3K/Akt signaling pathway
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Heterozygous p53-R280T mutations frequently occur in many nasopharyngeal carcinoma cell lines and nasopharyngeal carcinoma patients. However, the role of this mutation in the progression of nasopharyngeal carcinoma remains unclear. In this study, we successfully generated the tp53 knockout nasopharyngeal carcinoma (NPC) cells by CRISPR/Cas9-mediated genome editing and found that knockout of heterozygous tp53-R280T inhibited the proliferation of NPC cells significantly in vivo and in vitro. Mechanistic analyses indicated that heterozygous p53-R280T can activate the PI3K/Akt Signaling pathway in NPC cells. In conclusion, our findings provide a mechanistic insight into the role of heterozygous p53-R280T in NPC progression.
Overall design mRNA profiles of CNE2 (control) and KO CNE2 cells.
Contributor(s) Qin Z, Li Q, Xiao Z
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Apr 26, 2019
Last update date Jul 21, 2019
Contact name zhenqi qin
Organization name Xiangya Hospital, Central South University
Street address 87 Xiangya Road
City Changsha
ZIP/Postal code 410008
Country China
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (6)
GSM3737482 CNE2(WTa)
GSM3737483 CNE2(WTb)
GSM3737484 CNE2(WTc)
BioProject PRJNA539983
SRA SRP194002

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE130398_fpkm.txt.gz 1.2 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap