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Series GSE130395 Query DataSets for GSE130395
Status Public on Dec 17, 2019
Title Transcriptome profiling of prostate cancer cells on GLYATL1 knockdown
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The protein Glycine-N-Acyltransferase Like 1 (GLYATL1) is involved in detoxification of benzoate and other xenobiotics and is expressed in liver and kidney. Through In silico analysis of cancer gene expression profiling and transcriptome sequencing we revealed an overexpression of GLYATL1 in primary prostate cancer. Confirming these findings by immunohistochemistry we show that GLYATL1 is overexpressed in primary prostate cancer compared to metastatic prostate cancer and benign prostatic tissue. Low grade cancers had higher GLYATL1 expression compared to high grade prostate tumors. Our studies showed that GLYATL1 is upregulated upon androgen treatment in LNCaP prostate cancer cells which harbors ETV1 gene rearrangement. Furthermore, ETV1 knockdown in LNCaP cells showed downregulation of GLYATL1 suggesting potential regulation of GLYATL1 by ETS transcription factor ETV1. Transcriptome sequencing using the GLYATL1 knockdown prostate cancer cell lines LNCaP showed regulation of multiple metabolic pathways. In summary, our study characterizes the expression GLYATL1 in prostate cancer and explore its regulation mechanism. Future studies are needed to decipher the biological significance of these findings.
Overall design RNA from prostate cancer cells (22Rv1 and LnCAP) treated with GLYATL1 shRNA and non-targeting shRNA were sequenced. RNA-seq experiment is intended to identify genes and pathways altered on GLYATL1 knockdown.
Contributor(s) Eich M, Varambally S
Citation(s) 31376196
Submission date Apr 26, 2019
Last update date Dec 17, 2019
Contact name Sooryanarayana Varambally
Organization name University of Alabama
Department Pathology
Street address 1824 6th Avenue S
City Birmingham
State/province Alabama
ZIP/Postal code 35294-0007
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (4)
GSM3737427 22Rv1-GLYATL-sh1
GSM3737428 22Rv1-Scr
GSM3737429 LnCAP-GLYATL1-sh1
BioProject PRJNA539980
SRA SRP193999

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Supplementary file Size Download File type/resource
GSE130395_Matrix-file.txt.gz 629.2 Kb (ftp)(http) TXT
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