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Series GSE130243 Query DataSets for GSE130243
Status Public on Aug 14, 2019
Title Quantitative Analysis of Wild Type and scrib mutant Drosophila wing imaginal discs Transcriptomes at different time points
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: There are two goals of this study. Firstly, we want to figure out the transcriptomic differences between control and scrib¹ mutant wing imaginal discs. Secondly, we aim to investigate the transcriptomic changes as the scrib¹ mutant tumor develop over time.
Methods: Total RNA was used to generate cDNA libraries and 150bp paired-end sequencing on Illumina HiSeq platform were performed by Novogene. Cleaned raw reads were mapped to the reference genome using STAR and counts are generated by featuresCounts available in Subread package. Count normalization was performed using edgeR.
 
Overall design mRNA profiles of 4, 5-day wild type and 4, 5, 6, 8, 10, 12, 14-day scrib mutant wing imaginal discs were generated by Next Generation Sequencing, in triplicate or quadruplicate, using Illumina HiSeq.
 
Contributor(s) Ji T, Zhang L, Deng M, Yan Y
Citation(s) 31371383
Submission date Apr 24, 2019
Last update date Sep 18, 2019
Contact name Yan Yan
E-mail(s) drosophilaust@gmail.com
Organization name HKUST
Department LIFS
Street address Clear Water Bay
City Hong Kong
ZIP/Postal code 999077
Country China
 
Platforms (1)
GPL21306 Illumina HiSeq 4000 (Drosophila melanogaster)
Samples (34)
GSM3734358 WT_4d rep1
GSM3734359 WT_4d rep2
GSM3734360 WT_4d rep3
Relations
BioProject PRJNA534434
SRA SRP193697

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE130243_RAW.tar 3.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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