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Series GSE13015 Query DataSets for GSE13015
Status Public on Jul 01, 2009
Title Genomic Transcriptional Profiling Identifies a Blood Biomarker Signature for the Diagnosis of Septicemic Melioidosis
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Melioidosis is a severe infectious disease caused by Burkholderia pseudomallei, a gram-negative bacillus classified by the NIAID as a category B priority agent. Septicemia is the most common presentation of the disease with 40% mortality rate even with appropriate treatments. Faster diagnostic procedures are required to improve therapeutic response and survival rates. We have used microarray technology to generate genome-wide transcriptional profiles (>48,000 transcripts) of whole blood obtained from patients with septicemic melioidosis (n=32), patients with sepsis caused by other pathogens (n=31), and uninfected controls (n=29). Unsupervised analyses demonstrated the existence of a whole blood transcriptional signature distinguishing patients with sepsis from control subjects. The majority of changes observed were common to both septicemic melioidosis and sepsis caused by other infections, including genes related to inflammation, interferon-related genes, neutrophils, cytotoxic cells, and T cells. Finally, class prediction analysis identified a 37 transcript candidate diagnostic signature that distinguished melioidosis from sepsis caused by other organisms with 100% and 78% accuracy in training and independent test sets, respectively. This finding was confirmed by the independent validation set, which showed 80% prediction accuracy. This signature was highly enriched in genes coding for products involved in the MHC Class II antigen processing and presentation pathway. Transcriptional patterns of whole blood RNA distinguish patients with septicemic melioidosis from patients with sepsis caused by other pathogens. Once confirmed in a large scale trial this diagnostic signature might constitute the basis of a differential diagnostic assay.
 
Overall design Whole blood obtained from patients with sepsis caused by B.pseudomallei or other pathogens were collected. mRNA Expression level of each patient was determined and compared to mRNA expression level from uninfected control including patient who had recovered from melioidosis, patient diagnosed type 2 diabetes, and healthy donors suing microarray analysis.
 
Citation(s) 19903332, 33624743
Submission date Oct 02, 2008
Last update date Aug 31, 2021
Contact name Damien Chaussabel
E-mail(s) DChaussabel@benaroyaresearch.org
Organization name Baylor Institute for Immunology Research
Street address 3434 Live Oak
City Dallas
State/province TX
ZIP/Postal code 75204
Country USA
 
Platforms (2)
GPL6106 Sentrix Human-6 v2 Expression BeadChip
GPL6947 Illumina HumanHT-12 V3.0 expression beadchip
Samples (106)
GSM326090 M016
GSM326091 M017
GSM326092 I016
Relations
BioProject PRJNA112555

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Supplementary file Size Download File type/resource
GSE13015_RAW.tar 330.9 Mb (http)(custom) TAR (of XLS)
Raw data provided as supplementary file
Processed data included within Sample table

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