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Series GSE130054 Query DataSets for GSE130054
Status Public on Jul 09, 2019
Title Priming mobilization of hair follicle stem cells triggers permanent loss of regeneration after alkylating chemotherapy
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The maintenance of genetic integrity is critical for stem cells to ensure homeostasis and regeneration. Little is known about how adult stem cells respond to irreversible DNA damage, resulting in loss of regeneration in humans. Here, we establish a permanent regeneration loss model using cycling human hair follicles treated with alkylating agents: busulfan followed by cyclophosphamide. We unravel the underlying mechanisms by which hair follicle stem cells (HFSCs) lose their pool. Contrary to immediate destructive changes in rapidly proliferating hair matrix cells, quiescent HFSCs show unexpected massive proliferation after busulfan and then undergo large-scale apoptosis following cyclophosphamide. HFSC proliferation is activated through PI3K/Akt pathway, and depletion is driven by p53/p38-induced cell death. RNA-seq analysis shows that HFSCs experience mitotic catastrophe with G2/M checkpoint activation. Our findings indicate that priming mobilization causes stem cells to lose their resistance to DNA damage, resulting in permanent loss of regeneration after alkylating chemotherapy.
 
Overall design Global gene expression profiles of human hair follicle stem cells after alkylating chemotherapy were generated by paired-end sequencing with 100-base pair length on an Illumina HiSeq 2500 sequencer .
 
Contributor(s) Kim J, Ohn J, Kim S, Lee W, Kim J, Kwon O
Citation(s) 31455775
Submission date Apr 18, 2019
Last update date Sep 03, 2019
Contact name Jin Yong Kim
E-mail(s) kaiderin@naver.com
Phone 82-2-2072-1781
Organization name Seoul National University College of Medicine
Department Department of Dermatology
Street address 101 Daehak-ro, Jongno-gu
City Seoul
ZIP/Postal code 03080
Country South Korea
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (9)
GSM3731086 PCIA_Con_1
GSM3731087 PCIA_Con_2
GSM3731088 PCIA_Con_3
Relations
BioProject PRJNA533647
SRA SRP193016

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE130054_PCIA_RSEM_Gene_FPKM.xlsx 3.7 Mb (ftp)(http) XLSX
GSE130054_PCIA_RSEM_Gene_Raw_Count.xlsx 3.4 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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