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| Status |
Public on Apr 05, 2021 |
| Title |
Transcriptome analysis of stomatal lineage ground cells (SLGCs) |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Identify SLGC cell fate by transcriptomes
Methods: Cells expressing SLGC markers (BASLp:BRX-YFP, BASLp:myrBRX-YFP and myrBRXp:myrBRX-YFP) and stomatal commitment stage marker (MUTEp:MUTE-YFP) were collected by FACS. 9dpg plants were used for protoplasting and subjected to cell sorting. Around 20,000/sample were cellected and used for library preparation.
Results: SLGCs are renriched in mitotic genes. In addition, top candidates, DEK and MYB16 proteins function in SLGC cell divisions.
Conclusions: SLGCs are division competent cells and the coordination of cell cycle and transcriptional regulation though DEK is important for maintaining the transition (SLGC) state during stomatal development.
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| Overall design |
SLGC mRNA profiles of 9dpg seedlings were generated by deep sequencing, in duplicates, using Illumina HiSeq.
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| Contributor(s) |
Ho CK, Bergmann D |
| Citation(s) |
33875598 |
| |
| Submission date |
Apr 17, 2019 |
| Last update date |
May 19, 2021 |
| Contact name |
Chin-Min Kimmy Ho |
| Organization name |
Academia Sinica
|
| Department |
IPMB
|
| Lab |
Ho
|
| Street address |
No. 128 Sec2 Academia Rd
|
| City |
Taipei |
| ZIP/Postal code |
115 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA533237 |
| SRA |
SRP192828 |
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