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| Status |
Public on Jan 31, 2024 |
| Title |
Primary bone marrow stromal cells in plasma cell disorders |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Multiple myeloma (MM) and its premalignant precursor MGUS (monoclonal gammopathy of undetermined significance) are clonal plasma cell diseases that develop in the bone marrow (BM) and are dependent on microenvironmental interactions. Primary bone marrow stromal cells (BMSCs) are key players in the BM microenvironment, however, their role in MGUS/MM pathophysiology is not known. We therefore investigated potential disease-specific alterations in prospectively isolated BMSCs from MM, MGUS and healthy controls. Clear disease-related BMSC surface marker and gene expression differences were recorded, and deep sequencing identified shared MGUS/MM as well as MM-specific molecular signatures. Moreover, we identified genes that were deregulated in a disease-stage manner, thus reflecting the progression from normal to MGUS and MM. Analysis of primary BMSCs revealed disease-stage related protein and gene expression patterns, which provides novel insight into the stromal transitions and their functional implications for plasma cell disease development and progression.
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| Overall design |
RNA sequencing data from primary prospectively-isolated and bulk-sorted bone marrow stromal cells (BMSCs) defined as lin-CD45-CD71-CD235a-CD31-CD271+ from multiple myeloma (MM, n=10) and MGUS (n=5) patients in comparison with older healthy donor (n=7) BMSCs
Submitter declares that the raw data will be submitted to EGA because of patient privacy concerns.
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| Contributor(s) |
Zacharaki D, Soneji S, Scheding S |
| Citation missing |
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| |
| Submission date |
Apr 15, 2019 |
| Last update date |
Jan 31, 2024 |
| Contact name |
Shamit Soneji |
| Organization name |
BMC
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| Street address |
Solvegatan 19
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| City |
Lund |
| ZIP/Postal code |
22184 |
| Country |
Sweden |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA532852 |
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