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Series GSE129801 Query DataSets for GSE129801
Status Public on Jan 31, 2024
Title Primary bone marrow stromal cells in plasma cell disorders
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Multiple myeloma (MM) and its premalignant precursor MGUS (monoclonal gammopathy of undetermined significance) are clonal plasma cell diseases that develop in the bone marrow (BM) and are dependent on microenvironmental interactions. Primary bone marrow stromal cells (BMSCs) are key players in the BM microenvironment, however, their role in MGUS/MM pathophysiology is not known. We therefore investigated potential disease-specific alterations in prospectively isolated BMSCs from MM, MGUS and healthy controls. Clear disease-related BMSC surface marker and gene expression differences were recorded, and deep sequencing identified shared MGUS/MM as well as MM-specific molecular signatures. Moreover, we identified genes that were deregulated in a disease-stage manner, thus reflecting the progression from normal to MGUS and MM. Analysis of primary BMSCs revealed disease-stage related protein and gene expression patterns, which provides novel insight into the stromal transitions and their functional implications for plasma cell disease development and progression.
 
Overall design RNA sequencing data from primary prospectively-isolated and bulk-sorted bone marrow stromal cells (BMSCs) defined as lin-CD45-CD71-CD235a-CD31-CD271+ from multiple myeloma (MM, n=10) and MGUS (n=5) patients in comparison with older healthy donor (n=7) BMSCs
Submitter declares that the raw data will be submitted to EGA because of patient privacy concerns.
 
Contributor(s) Zacharaki D, Soneji S, Scheding S
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Submission date Apr 15, 2019
Last update date Jan 31, 2024
Contact name Shamit Soneji
Organization name BMC
Street address Solvegatan 19
City Lund
ZIP/Postal code 22184
Country Sweden
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (22)
GSM3722279 HD-10
GSM3722280 HD-3
GSM3722281 HD-4
Relations
BioProject PRJNA532852

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129801_RAW.tar 3.2 Mb (http)(custom) TAR (of TXT)
Processed data provided as supplementary file
Raw data not provided for this record

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