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| Status |
Public on Apr 12, 2019 |
| Title |
RNA sequence data in whole cell extracts of differentiated human podocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Sphingomyelin phosphodiesterase acid-like 3b (SMPDL3b) is a lipid raft enzyme that regulates plasma membrane (PM) fluidity. Here we report that SMPDL3b excess, as observed in podocytes in diabetic kidney disease (DKD), impairs insulin receptor isoform B-dependent pro-survival insulin signaling by interfering with insulin receptor isoforms binding to caveolin-1 in PM. SMPDL3b excess affects the production of active sphingolipids resulting in decreased ceramide-1-phosphate (C1P) content as observed in human podocytes in vitro and in kidney cortexes of diabetic db/db mice in vivo. Podocyte-specific Smpdl3b deficiency in db/db mice is sufficient to restore kidney cortex C1P content and to protect from DKD. Exogenous administration of C1P restores IR signaling in vitro and prevents established DKD progression in vivo. Taken together, we identified SMPDL3b as a modulator of insulin signaling and demonstrated that supplementation with exogenous C1P may represent a lipid therapeutic strategy to treat diabetic complications such as DKD.
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| Overall design |
RNA sequence data in whole cell extracts of differentiated human podocytes
RNA from Ctrl and SMPDL3b OE podocytes was isolated and analyzed for differences in the mRNA expression profile
RNA extraction, Preparation and sequencing RNA libraries, Illumina Sequencing RNA Data Analysis
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| |
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| Contributor(s) |
Mallela SK, Mitrofanova A |
| Citation(s) |
31217420 |
| |
| Submission date |
Apr 11, 2019 |
| Last update date |
Jul 01, 2019 |
| Contact name |
Shamroop kumar Mallela |
| E-mail(s) |
shamroop4@gmail.com
|
| Organization name |
University of Miami
|
| Department |
Medicine
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| Lab |
Nephrology
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| Street address |
1580 NW 10th Ave
|
| City |
Miami |
| State/province |
Florida |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA532413 |
| SRA |
SRP192089 |
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