Expression profiling by high throughput sequencing
Summary
We performed genome-wide transcriptome profiling in stable Kmt2d-/- (bi-allelic deletion of the catalytic SET domain) and Kmt2d+/+ ATDC5 chondrocyte cell lines 7 days after induction of differentiation and in stable Kmt2d-/- and Kmt2d+/+ undifferentiated ATDC5 cells.
Overall design
RNA-seq was performed on two (Kmt2d-/-) and three (Kmt2d+/+) biological replicates for each differentiation state (chondrocytes vs undifferentiated cells); each biological replicate is a distinct clonal cell line. Two technical replicates were performed for each cell line. Technical replicates were the same libraries run on different flow cells.
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