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| Status |
Public on Jan 27, 2021 |
| Title |
RNA-seq analysis of the effects of forskolin treatment on human primary adipocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Analysis of forskolin-stimulated cAMP signalling on adipocyte metabolism at gene expression level. Results provide important information of the response of adipocytes to cAMP signaling (a cold-conditioned mimc), such as specific metabolic genes, up- or down-regulated that control expression of putative adipokines.
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| Overall design |
Human white primary preadipocytes (PromoCell, Heidelberg, Germany) were seeded (10,000-15,000 cells/cm2) and grown to confluence (37°C, 5% CO2) in preadipocyte growth medium (0.05ml/ml fetal calf serum, 0.004ml/ml endothelial cell growth supplement, 10ng/ml epidermal growth factor, 1µg/ml hydrocortisone, 90µg/ml heparin). Preadipocytes were differentiated according to supplier’s instructions. Briefly, growth medium was replaced by differentiation media (8µg/ml d-biotin, 0.5µg/ml insulin, 400ng/ml dexamethasone, 44µg/ml isobutylmethylxanthine, 9ng/ml L-thyroxine, 3µg/ml ciglitazone) for 48 hours. Differentiation medium was replaced with adipocyte nutrition media (0.03ml/ml fetal calf serum, 8µg/ml d-biotin, 0.5µg/ml insulin, 400ng/ml dexamethasone) for 12 days until cells were fully differentiated. Media was changed every 48 hours. Human white primary adipocytes were treated with 1 μM forskolin on days 10 - 12 of differentiation or remained untreated (control). Total RNA was then isolated.
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| Contributor(s) |
Roberts LD |
| Citation missing |
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| Submission date |
Apr 01, 2019 |
| Last update date |
Jan 30, 2021 |
| Contact name |
Lee Roberts |
| E-mail(s) |
L.D.Roberts@leeds.ac.uk
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| Organization name |
University of Leeds
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| Department |
LICAMM
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| Street address |
Level 7 LIGHT Laboratories, University of Leeds
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| City |
Leeds |
| State/province |
Yorkshire |
| ZIP/Postal code |
LS2 9JT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA530238 |
| SRA |
SRP190042 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE129153_RAW.tar |
1.0 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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